先天性人巨细胞病毒感染对乙肝疫苗免疫效果的影响

发布时间：2018-06-13 08:12

本文选题：人巨细胞病毒 + 先天性感染　；参考：《临床儿科杂志》2014年02期

【摘要】：目的探讨先天性人巨细胞病毒(HCMV)感染对乙型肝炎(乙肝)疫苗免疫效果的影响。方法孕鼠腹腔注射HCMV悬液,以其子鼠为先天性HCMV感染模型,以健康大鼠的子鼠作为对照组。各组子鼠分别于生后1、3、5周接种乙肝疫苗,并于3、5、7、11周心脏采血,应用酶联免疫分析法检测各组子鼠血清乙肝表面抗体(HBsAb)滴度。结果随接种次数增加,两组子鼠血清HBsAb滴度均呈逐渐增加趋势,接种完成后随时间延长又有不同程度下降,两组子鼠血清HBsAb滴度随时间变化的差异均有统计学意义(P均0.001)。在各个时间点(3、5、7、11周),先天性HCMV感染组子鼠的血清HBsAb滴度均低于对照组,差异有统计学意义(P均0.01)。结论先天性HCMV感染可减弱乙肝疫苗免疫效果。
[Abstract]:Objective to investigate the effect of congenital human cytomegalovirus (HCMV) infection on hepatitis B vaccine. Methods pregnant rats were injected with HCMV suspension intraperitoneally, and their offspring were used as the model of congenital HCMV infection, and the healthy rats were used as the control group. The offspring of each group were inoculated with hepatitis B vaccine at 1 day and 3 weeks after birth, and their heart blood was collected at 7 and 11 weeks after birth. Elisa was used to detect the serum HBsAg Ab titer of each group. Results with the increase of inoculation times, the serum HBsAg Ab titers of the two groups increased gradually, and then decreased with time after inoculation. There were significant differences between the two groups in the changes of serum HBsAg Ab titers with time (P 0.001). At each time point, the serum HBsAg titers of the infantile mice of congenital HCMV infection group were lower than those of the control group, and the difference was statistically significant (P < 0.01). Conclusion congenital HCMV infection can attenuate the immune effect of hepatitis B vaccine.
【作者单位】：潍坊医学院;山东大学附属济南市中心医院;
【分类号】：R392.1

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