人毛乳头细胞HSPC016基因的克

发布时间：2018-06-13 17:15

本文选题：毛囊 + 毛囊生长周期　；参考：《第三军医大学》2005年硕士论文

【摘要】：毛囊是重要的皮肤附属器官,是毛发的主体。毛发具有调节体温、排泄代谢物、缓冲外界机械压力等重要生理功能,对人类而言同时还有美学、装扮、特征辨认等社会学意义。因毛囊异常生长所致的多毛或少毛不仅会影响毛发生理功能的正常发挥而且正在或可能将要给越来越多的患者造成心理压力,直接影响患者的健康和生活质量。因此,了解毛囊生长的确切机制、调控原理及探讨临床诊治毛囊疾病的方法与措施已成必然。多数学者认为毛乳头在毛囊的形态学发生和成年毛囊的周期性生长与调控中起主导作用,毛乳头细胞是毛囊研究的中心环节之一。造血干细胞(hematopoietic stem/progenitor cell,HSPC)相关基因HSPC016 是我国复旦大学学者在人造血干细胞中发现的新基因,而我们首次发现了HSPC016 基因作为上调表达基因在凝集性生长状态人毛乳头细胞差异表达cDNA 文库中的表达。对于一个新基因而言,获取其重组蛋白,是进一步对基因功能进行深入研究所必需的。毛囊的明显特性是自我更新和周期性生长。完整的毛囊生长周期包括生长期(anagen)、退行期(catagen)、休止期(telogen)。毛囊的胚胎发育和周期性生长主要依靠来源于毛囊上皮与间质毛乳头(dermal papillae, DP)的相互作用。毛乳头是真皮成分突入毛球内形成的特殊结构,为含血管和神经的间充质成分。毛乳头细胞(dermal papilla cells, DPC)是组成毛乳头的唯一细胞,在毛囊的胚胎发生和周期性生长过程中起着十分重要的作用,一方面提供毛囊生长和分化必需的信号因子和营养支持,另一方面还决定毛囊的分化方向。因此,毛乳头细胞是毛囊研究的中心环节之一。毛乳头是一个可诱导的结构,它能发送或接受信号,从而影响毛囊的发育和周期性生长。通过对小鼠/大鼠模型的研究,一些与毛囊生长周期有关的调节分子如成纤维细胞生长因子(fibobalast growth factors, FGFs)、骨形态发生蛋白(bone morphogenetic proteins, BMPs)、刺猬蛋白(sonic hedgehog, Shh)、神经营养因子(neurotrophins)、血小板衍生生长因子(platelet derived growth factor, PDGF)等已相继被鉴定出来。组成毛囊的细胞如DPC、外根鞘细胞(out root sheath, ORS)、毛母质细胞等可分泌这些生长因子及其受体;而且就单一细胞因子而言,其产生和分泌都与毛囊周期的特定时相有关。但是,关于这些细胞因子调节毛囊胚胎发生和毛囊生长周期的具体分子机制如通过什
[Abstract]:Hair follicles are important skin appendages and the main body of hair. Hair has important physiological functions, such as regulating body temperature, excreting metabolites, cushioning external mechanical pressure and so on. It also has sociological significance for human being, such as aesthetics, dress up, characteristic recognition and so on. The abnormal growth of hair follicles can not only affect the normal play of hair physiological function, but also may cause psychological stress to more and more patients, and directly affect the health and quality of life of patients. Therefore, it is necessary to understand the exact mechanism of hair follicle growth, regulation principle and clinical diagnosis and treatment of hair follicle disease. Most scholars believe that dermal papilla plays a leading role in the morphology of hair follicles and the periodic growth and regulation of adult hair follicles, and dermal papilla cells are one of the central links of hair follicle research. Hematopoietic stem/progenitor cell HSPC-associated gene HSPC016 is a new gene found by scholars of Fudan University in China. The expression of HSPC016 gene in the cDNA library of differential expression of human dermal papilla cells in agglutinative growth state was first found. For a new gene, it is necessary to obtain its recombinant protein for further study of gene function. Hair follicles are characterized by self-renewal and periodic growth. The complete hair follicle growth cycle includes the growth stage anagenase, the receding stage and the rest stage. The embryonic development and periodic growth of hair follicles mainly depend on the interaction between hair follicle epithelium and dermal papilla (DP). The dermal papilla is a special structure formed by the dermis penetrating into the hair bulb. It is a mesenchymal component containing blood vessels and nerves. Dermal papilla cells (DPCs) are the only cells that make up the dermal papilla. They play an important role in the embryogenesis and periodic growth of hair follicles. On the one hand, they provide the necessary signal factors and nutritional support for the growth and differentiation of hair follicles. On the other hand, it also determines the direction of hair follicle differentiation. Therefore, dermal papilla cells are one of the central links in the study of hair follicles. Dermal papilla is an inducible structure, which can send or receive signals, thus affecting the development and periodic growth of hair follicles. By studying the mouse / rat model, Some regulatory molecules related to hair follicle growth cycle, such as fibroblast growth factor (FGFs), bone morphogenetic protein (BMP), hedgehog protein (Sonic hedgehog), Shhmong, neurotrophins (neurotrophins) and platelet-derived growth factor (platelet derived growth factor, PDGFs), etc. Have been identified one after another. These growth factors and their receptors can be secreted by hair follicle cells such as DPCs, outer root sheath cells, root sheathes, orthorhombic cells and hair mother cells, and in the case of single cytokines, their production and secretion are related to the specific phase of hair follicle cycle. However, specific molecular mechanisms for these cytokines to regulate hair follicle embryogenesis and hair follicle growth cycle, such as through what
【学位授予单位】：第三军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346

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