不同浓度粒巨细胞集落刺激因子及白介素-4对树突状细胞体外诱导培养的影响

发布时间：2018-06-14 06:05

本文选题：树突细胞 + 细胞培养技术　；参考：《中国临床保健杂志》2014年02期

【摘要】：目的探讨不同浓度小鼠粒/巨噬细胞集落刺激因子(GM-CSF)和白介素-4(IL-4)对小鼠骨髓源性树突状细胞培养的影响,探索树突状细胞诱导培养的适宜条件。方法采用细胞裂解法分离得到Balb/c小鼠骨髓单个核样细胞,分为多组,分别加入不同浓度GM-CSF和IL-4,并设置空白对照组,共培养7 d,每天观察各组细胞形态,隔天拍照。第7天收集细胞,使用流式细胞法检测细胞表面树突状细胞标志分子CD11c的表达率。结果低浓度GM-CSF及IL-4可培养出高纯度小鼠骨髓源性树突状细胞,高浓度GM-CSF及IL-4可导致细胞死亡,GM-CSF和IL-4的合理细胞诱导浓度范围分别为5~100μg/L和2.5~50μg/L。结论合适浓度的GM-CSF及IL-4可诱导小鼠骨髓单个核样细胞向树突状细胞分化,获得大量树突状细胞。
[Abstract]:Objective to investigate the effects of different concentrations of mouse granulocyte / macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) on the culture of murine bone marrow-derived dendritic cells (BMSCs). Methods Balb / c mice bone marrow mononuclear cells were isolated by cell lysis method. The cells were divided into two groups: different concentrations of GM-CSF and IL-4. The cells were cultured in blank control group for 7 days. The morphology of each group was observed every day and photographed the next day. On the 7th day, the expression rate of CD11c, a dendritic cell marker, was detected by flow cytometry. Results High purity murine bone marrow-derived dendritic cells could be cultured with low concentration of GM-CSF and IL-4. The reasonable concentration of GM-CSF and IL-4 could induce cell death in the range of 5 ~ 100 渭 g / L and 2.5 ~ 50 渭 g / L, respectively. Conclusion the suitable concentration of GM-CSF and IL-4 can induce mouse bone marrow mononuclear cells to differentiate into dendritic cells and obtain a large number of dendritic cells.
【作者单位】：安徽医科大学附属省立医院、安徽省立医院呼吸内科;
【基金】：2013年度安徽省自然科学基金项目(1308085MH115)
【分类号】：R392

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