高交感活性诱导的大鼠心肌损伤模型中氧化应激的受体调控机制

发布时间：2018-06-14 06:26

本文选题：高交感活性 + 氧化性应激　；参考：《中国病理生理杂志》2014年06期

【摘要】：目的:研究高交感活性诱发大鼠心肌损伤的氧化应激受体调控机制。方法:Sprague-Dawley(SD)大鼠随机分为对照组、模型组、普萘洛尔(Pro)组、哌唑嗪(Praz)组、普萘洛尔+哌唑嗪(Pro+Praz)组、维生素E(VE)组及普萘洛尔+哌唑嗪+维生素E(Pro+Praz+VE)组,除对照组外其余各组均腹腔注射去甲肾上腺素(NE)复制高交感活性引起的心肌损伤模型,同时灌胃给予相应药物,连续给药16 d,期间监测各组动物体重的变化。16 d后进行心室重构指标(心指数和羟脯氨酸含量)、病理组织学检查、氧化/抗氧化指标(MDA、SOD、CAT、GSH-Px和T-AOC)和能量代谢指标(Na+-K+ATPase和Ca2+-Mg2+ATPase)分析。结果:从第9天开始,模型组动物体重与对照组的比较差异有统计学意义(P0.05),心指数和左心室肥厚明显增加,氧化/抗氧化和能量代谢障碍;Pro、Praz、Pro+Praz和VE各组均出现不同程度的动物体重、心指数、左心室肥厚和氧化/抗氧化失衡的改善;Pro、Praz和Pro+Praz能明显升高左心室Na+-K+ATPase和Ca2+-Mg2+ATPase的活性,Pro+Praz作用最明显(P0.05)。结论:肾上腺素受体依赖是高交感活性诱导心肌氧化应激损伤的重要途径。
[Abstract]:Aim: to study the regulatory mechanism of oxidative stress receptor (OSR) on myocardial injury induced by high sympathetic activity in rats. Methods SD rats were randomly divided into three groups: control group, model group, propranolol group, prazidine group, prozidine group, vitamin E (VEV) group and propranolprazin (Pro Praz) group. The myocardial injury model induced by hypersympathetic activity was established by intraperitoneal injection of norepinephrine (NE) in all the other groups except the control group, and the corresponding drugs were given intragastrically. The changes of body weight in each group were monitored for 16 days. The indexes of ventricular remodeling (cardiac index, hydroxyproline content, histopathology) were measured. GSH-Px and T-AOC), Na -K ATPase and Ca 2 mg 2 ATPasewere determined by oxidation / oxidation index (MDA-SODX and T-AOC). Results: from the 9th day, the body weight of the model group was significantly different from that of the control group (P 0.05), and the cardiac index and left ventricular hypertrophy were significantly increased. Different levels of animal body weight and cardiac index were found in Propranzia Praz and VE groups. Improvement of left Ventricular hypertrophy and Oxidation / Antioxidant imbalance Pro-Praz and Pro Praz could significantly increase the activity of left ventricular Na -K ATPase and Ca 2 mg 2 ATPase. Conclusion: adrenergic receptor dependence is an important pathway of myocardial oxidative stress injury induced by high sympathetic activity.
【作者单位】：贵阳医学院药理研究室;贵阳医学院附属医院心内科;
【基金】：国家自然科学基金资助项目(No.81173586) 教育部新世纪优秀人才支持计划(No.NCET-13-0747) 贵州省优秀科技教育省长专项资金资助项目(黔科教办[2011]28号) 贵州省社发攻关项目(黔科合SY字[2011]3011号) 贵州省科技厅科技攻关项目(黔科合SY字[2012]3085号)
【分类号】：R363

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