低氧及运动对骨骼肌中低氧诱导因子影响的实验研究

发布时间：2018-06-16 01:14

  本文选题：高原 + 低氧　； 参考：《第一军医大学》2005年博士论文

【摘要】：目的:研究低氧及低氧复合运动情况下大鼠体重、骨骼肌显微、超微结构及低氧诱导因子发生变化的规律,探讨在低氧条件下提高机体低氧习服能力的途径和可能的机制。 方法:分别建立大鼠低氧及正常氧情况下运动模型:雄性SD大鼠80只,随机分为4组,即常氧安静组(A)、常氧运动组(B)、低氧安静组(C)、低氧运动组(D)。大鼠运动采用跑台模型,速度设定为25m/min,检测在第3天(急性低氧)、第7天、第10天与第14天(慢性低氧)各组大鼠体重(W)变化,光镜和电镜分别观察骨骼肌显微和超微结构的改变,对骨骼肌中低氧诱导因子-1(HIF-1)的表达运用实时荧光PCR(fluorescence quantitative PCR FQ-PCR)、表面加强激光解析电离化芯片技术(Surface-enhanced LaserDesorption/Ionization SELDI)进行定量分析;采用2×4×2析因分析,分组变量分别为氧含量、运动与否以及时间,其中氧含量为2个水平:正常氧和低氧;运动情况有2个水平:安静与运动;时间有4个水平,分别为3天、7天、10天、14天。分别研究低氧及运动的不同阶段对骨骼肌形态结构的影响以及骨骼肌中低氧诱导因子表达的变化规律,分析其相互作用,探讨骨骼肌结构、功能改变的发生机制及演变过程,寻找在低氧条件下促进低氧习服能力的途径,探讨其发生的可能的机制。 结果:单纯低氧组大鼠体重在试验前后有所降低,而正常氧安静组、常氧运动组及低氧运动组大鼠体重均有不同程度的增加,与低氧安静组比较有显著意义(p0.05)。在肌肉形态学方面,单纯低氧组大鼠急性期光镜下肌纤维形态尚正常,电镜下已出现线粒体体积增大、肿胀,嵴稍肿胀、嵴内腔扩大,嵴紊乱;在慢性期光镜下出现肌纤维萎缩,电镜下可见线粒体轻度肿胀,水性变及空泡化,
[Abstract]:Aim: to study the changes of body weight, skeletal muscle microstructure, ultrastructure and hypoxia inducible factors in hypoxic and hypoxic combined exercise rats, and to explore the ways and possible mechanisms of enhancing hypoxic acclimatization under hypoxic conditions. Methods: 80 male Sprague-Dawley (SD) rats were randomly divided into four groups: normal oxygen quiet group, normoxic exercise group, hypoxia quiet group and hypoxic exercise group. The rats used a treadmill model with a speed of 25 m / min. The changes of body weight were measured on day 3 (acute hypoxia, day 7, day 10 and day 14 (chronic hypoxia). The changes of microstructure and ultrastructure of skeletal muscle were observed by light microscope and electron microscope respectively. The expression of hypoxia inducible factor-1 (HIF-1) in skeletal muscle was quantitatively analyzed by real-time fluorescence quantitative FQ-PCRN, surface-enhanced laser desorption / ionization chip technique (Surface-enhanced LaserDesorptionIonization SELDI). Using 2 脳 4 脳 2 factorial analysis, the grouped variables are oxygen content, exercise or not, and time, in which oxygen content is 2 levels: normal oxygen level and hypoxia level; exercise condition has 2 levels: quiet and exercise; time has 4 levels. They were 3 days, 7 days, 10 days and 14 days. The effects of hypoxia and exercise on skeletal muscle morphology and the expression of hypoxia inducible factor in skeletal muscle were studied, and the mechanism and evolution of skeletal muscle structure and function were discussed. To find ways to promote hypoxic acclimatization under hypoxia conditions and explore the possible mechanism of its occurrence. Results: the body weight of rats in hypoxic group was decreased before and after the experiment, but the body weight of rats in normal oxygen quiet group, normoxic exercise group and hypoxic exercise group increased in varying degrees, which was significantly higher than that in hypoxic quiet group (P 0.05). In terms of muscle morphology, the morphology of muscle fibers was normal under light microscope in the acute phase of hypoxic rats. Mitochondria volume increased, swelling, cristae slightly swollen, cristal cavity enlarged and cristal disorder appeared under electron microscope. Myofibril atrophy was observed under the light microscope in chronic phase, slight swelling of mitochondria, waterborne degeneration and vacuolation were observed under electron microscope.
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R363;R87

【引证文献】

中国期刊全文数据库 前1条

1 谢康玲;刘遂心;蔡颖;张文亮;;长期中等强度运动对小鼠骨骼肌HIF-1α mRNA的表达及葡萄糖转运的影响[J];中国康复医学杂志;2012年06期

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