冰冻组织芯片的研制及可行性探讨与组织芯片中Skp2和相关蛋白的表达及其生物学意义

发布时间：2018-06-16 01:45

本文选题：冰冻组织芯片 + 模具　；参考：《天津医科大学》2006年硕士论文

【摘要】：目的: 应用自制模具进行冰冻组织芯片的研制及可行性探讨,为简单、经济、规范地进行蛋白及基因原位分析提供一种新方法。方法: 收集天津医科大学总医院、天津市第一中心医院及天津市胸科医院2004年3月到2005年3月新鲜原发性肺癌手术标本19例,癌旁正常肺组织5例。应用自制模具制备48点阵冰冻组织芯片。并经常规HE及EMA免疫组化染色,对其在形态学及基因表达分析方面的可行性进行评价。结果: 1.冰冻组织芯片OCT模块和HE切片的观察:模块上的组织芯排列基本整齐,组织芯无缺损,芯与芯之间无挤压扭曲现象;HE切片镜下每一组织芯中均可见具有诊断意义的肿瘤组织结构,所有组织中,细胞核呈蓝色,细胞质呈红色,颜色鲜艳,核浆对比清晰,细胞的形态结构基本清楚。 2.免疫组化染色观察:镜下组织芯有2点不完全脱片,未脱片的组织形态完好,背景清楚,胞浆不同程度表达EMA,呈棕黄色颗粒状。 3.冰冻组织芯片EMA免疫组化染色结果与普通冰冻切片免疫组化结果比较,符合率为94.7%(18/19),二者比较差异无统计学意义(p0.05)。结论: 1.我们冰冻组织芯片的研制方法是可行的,完全可以用于形态学观察和蛋白表
[Abstract]:Objective: to study the feasibility of frozen tissue microarray by using self-made mould, and to provide a new method for protein and gene in situ analysis in a simple, economical and standard way. Methods: 19 fresh specimens of primary lung cancer were collected from Tianjin Medical University General Hospital, Tianjin first Central Hospital and Tianjin chest Hospital from March 2004 to March 2005. There were 5 cases of normal lung tissue adjacent to cancer. A 48-lattice frozen tissue chip was fabricated by using a self-made mold. The feasibility of morphological and gene expression analysis was evaluated by routine HE and EMA immunohistochemical staining. Results: 1. Observation of frozen tissue chip Oct module and HE section: the tissue core was arranged neatly on the module, and there was no defect in the tissue core. The tumor tissue structure with diagnostic significance was found in each tissue core under HE section. In all tissues, the nucleus was blue, the cytoplasm was red, the color was bright, and the nuclear and cytoplasm were clearly contrasted. The morphology and structure of cells are basically clear. 2. Immunohistochemical staining showed that there were 2 points of incomplete strip in the tissue core under microscope. The tissue morphology of the tissue was intact and the background was clear. The cytoplasm expressed EMA in different degree and was brown granular. The results of frozen tissue microarray EMA immunohistochemical staining were compared with those of ordinary frozen sections. The coincidence rate was 94.7% (18 / 19). There was no significant difference between the two groups (p 0.05). Conclusion: 1. It is feasible to develop the frozen tissue microarray, which can be used for morphological observation and protein table.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R361

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