河南地区人源隐孢子虫生物学特性的实验研究

发布时间：2018-06-16 11:46

本文选题：隐孢子虫 + PCR　；参考：《郑州大学》2006年硕士论文

【摘要】：背景和目的隐孢子虫病(Cryptosporidiosis)是一种全球性的人兽共患寄生虫病，有着重要公共卫生意义。隐孢子虫可寄生于禽类、哺乳类、爬行类和两栖类等170种以上的人和动物。在人类患者主要引起腹泻和呼吸道症状，艾滋病病人和儿童中的感染率分别高达48％和17.5％，可导致严重的水样腹泻甚至危及生命，免疫功能正常者也能感染出现急性自限性腹泻。该病已被列为引起人类最常见的6种腹泻疾病之一。本文对河南地区3株人源隐孢子虫分离株进行了形态学测定、动物交叉感染试验、致病性和组织病理研究，并进行了PCR和基因进化树的构建，来确定3个隐孢子虫分离株的生物学特性。对于隐孢子虫病的深入研究，有赖于其动物模型的建立。3个隐孢子虫分离株均能成功感染免疫力低下及免疫力正常的小白鼠。在3个分离株中，1号和2号分离株感染小白鼠后出现的临床症状和病理变化基本一致，，且明显严重于3号分离株对小鼠的感染。此外1号和2号分离株感染小白鼠后的排隐孢子虫卵囊规律几乎一致，但二者与3号分离株感染小白鼠后的排隐孢子虫卵囊规律差别较大。在寄生部位上3者也有明显的区别，1号、2号分离株主要寄生于小白鼠的回肠，3号分离株主要寄生于小白鼠的十二指肠。只有3号分离株能够成功感染雏鸡，并且3号分离株能够使试验雏鸡出现严重的临床症状和病理变化。在寄生部位上，3号分离株主要寄生于雏鸡的回肠，这明显区别于感染小白鼠时的十二指肠。结果表明1、2号分离株为同一个种属，3号分离株为一个种属。
[Abstract]:Background and objective Cryptosporidiosism (Cryptosporidiosism) is a global zoonotic parasitic disease, which has important public health significance. Cryptosporidium can parasitize more than 170 species of people and animals, such as birds, mammals, reptiles and amphibians. In human patients, the main causes of diarrhea and respiratory symptoms, AIDS patients and children with infection rates as high as 48% and 17.5%, can lead to serious watery diarrhea and even life-threatening. People with normal immune function can also be infected with acute self-limited diarrhea. The disease has been listed as one of the six most common diarrhoeal diseases. In this paper, three human Cryptosporidium isolates in Henan Province were studied by morphological analysis, animal cross infection test, pathogenicity and histopathology, and the construction of PCR and gene evolution tree. To determine the biological characteristics of three Cryptosporidium isolates. The further study of Cryptosporidiosis depends on the establishment of animal models. All three Cryptosporidium isolates can successfully infect mice with low immunity and normal immunity. The clinical symptoms and pathological changes of mice infected with 1 and 2 strains were basically consistent, and were significantly more serious than the infection of strain 3 in mice. In addition, the oocysts of Cryptosporidium oocysts infected by isolates 1 and 2 were almost the same, but the rules of oocysts of Cryptosporidium were different from those of strain 3. There were also significant differences in the parasitic sites of the 3 isolates. Isolates 1 and 2 were mainly parasitic on the ileum of the mice and isolates 3 were mainly parasitic on the duodenum of the mice. Only strain 3 could infect chicks successfully, and strain 3 could cause serious clinical symptoms and pathological changes in experimental chicks. In the parasitic site, isolate 3 was mainly parasitic on the ileum of the chicks, which was obviously different from the duodenum of the infected mice. The results showed that strain 1 and 2 belonged to the same genus, and strain 3 belonged to one genus.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R382

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