绿原酸、异绿原酸对烟曲霉生物膜抑制作用的体外研究

发布时间：2018-06-17 05:59

  本文选题：烟曲霉 + 生物膜　； 参考：《中国感染与化疗杂志》2014年04期

【摘要】：目的通过在体外复制烟曲霉的生物膜模型,研究绿原酸(chlorogenic acid,CRA)、异绿原酸(isochlorogenic acid,IRC)对生物膜的影响。方法选取临床分离的烟曲霉构建体外生物膜模型,微量液体稀释法测定最低抑菌浓度(MIC),将菌株分为空白对照组、CRA实验组(1 024 mg/L、512 mg/L、256 mg/L),IRC实验组(1 000 mg/L、500 mg/L、250 mg/L),用扫描电子显微镜(scanning electron microscopy,SEM)、激光共聚焦扫描显微镜(laser confocal scanning microscope,CLSM)定性观察生物膜;结晶紫染色法半定量生物膜量。结果 37℃培养24 h烟曲霉形成早期生物膜,48 h形成成熟期生物膜。SEM及CLSM观察到实验组烟曲霉生物膜均较其相应空白对照组空洞、稀疏,但菌丝未见明显减少,死活菌数也无明显差别。CRA、IRC实验组早期结晶紫半定量结果为1.05±0.19、1.14±0.26、0.99±0.14、1.39±0.06、1.41±0.06、1.60±0.04,均少于空白对照组1.91±0.17,差异具有统计学意义(P0.05);CRA、IRC实验组晚期结晶紫半定量结果为2.25±0.05、2.27±0.05、2.31±0.03、2.26±0.02、2.27±0.02、2.29±0.04,少于空白对照组2.36±0.01,差异具有统计学意义(P0.05)。结论CRA、IRC对体外烟曲霉生物膜形成有抑制作用。
[Abstract]:Objective to study the effect of Lv Yuan acid chlorogenic acidogenic acid on biofilm by replicating the biofilm model of Aspergillus fumigatus in vitro. Methods an in vitro biofilm model was established by clinical isolation of Aspergillus fumigatus. Microliquid dilution method was used to determine the minimum inhibitory concentration (MIC), and the strains were divided into two groups: the control group: 1 024 mg / L 1 024 mg / L 512mg / L 256mg / L ~ 256mg / L ~ (-1). The biofilm was observed qualitatively by scanning electron microscope (SEM) and laser confocal scanning microscopeCLSM3 (1 000 mg / L ~ (- 500) mg 路L ~ (-1), scanning electron microscope (SEM), scanning electron microscope (SEM) and laser confocal scanning microscopeCLSM (laser confocal scanning microscope). Semi-quantitative biofilm volume by crystal violet staining. Results the biofilm of Aspergillus fumigatus was formed at 37 鈩，

本文编号：2029986