影响人囊胚形成因素的研究与人胚胎干细胞建系的初步探讨

发布时间：2018-06-17 18:31

  本文选题：囊胚 + 内细胞团　； 参考：《天津医科大学》2006年硕士论文

【摘要】：我国大部分生殖中心在行体外受精-胚胎移植(in vitro fertilization,IVF-ET)时通常应用D_3胚胎进行移植,然而囊胚移植更符合生理过程,可减少胚胎移植的数目,降低并发症的发生。为此,我们通过D_3剩余废弃胚胎的序贯培养得到了一定数目的囊胚,分析了影响人囊胚形成的因素,并且利用得到的囊胚在人胚胎肺组织成纤维细胞(human embryonic pulmonary fibroblasts,HEPFs)饲养层上对人胚胎干细胞(human embryonic stem cells,hESCs)建系进行了初步探讨。 研究分为三部分:第一部分,影响人囊胚形成因素的研究;第二部分,饲养层的制备与处理;第三部分,人胚胎干细胞建系的初步探讨。 我们将D_3移植后剩余无冻存价值的胚胎采用序贯培养的方法培养到D_(5-7),观察囊胚形成的情况及囊胚的质量,分析了D_3胚胎的细胞数和质量分级与囊胚及优质囊胚形成之间的关系,给囊胚移植提供理论支持。我们共得到98位患者D_3移植后剩余无冻存价值的203个胚胎,在患者签署知情同意书后进入本实验。将胚胎于D_3转移至G2.3中,每天观察胚胎发育的情况直至受精D_(5-7)并且对囊胚进行分级。共有51个囊胚形成,囊胚形成率为25.12%,优质囊胚有14个,占所有囊胚的27.45%,全部由Ⅱ~+级以上胚胎形成。32位有囊胚形成的患者有16人获临床妊娠,临床妊娠率为50%,高于66位无囊胚形成患者28.79%的妊娠率(P0.05)。通过分析,我们得出结论:D_3胚胎细胞数与囊胚形成率呈显著正相关关系,Ⅰ-Ⅱ~+级胚胎比Ⅲ-Ⅳ级胚胎囊胚形成率、优质囊胚形成率高。
[Abstract]:In most reproductive centers in China, D3 embryos are usually used for in vitro fertilization and embryo transfer (IVF-ETT). However, blastocyst transfer is more suitable for physiological process, which can reduce the number of embryo transfer and reduce the incidence of complications. For this reason, we obtained a certain number of blastocysts through sequential culture of the remaining discarded embryos of DST3, and analyzed the factors affecting the formation of human blastocysts. The blastocysts were used to establish human embryonic pulmonary embryonic stem cells hESCs on the feeder layer of human embryonic pulmonary fibroblast cells (HEPFs). The research is divided into three parts: the first part is the study of the factors affecting the formation of human blastocyst; the second part is the preparation and treatment of feeder layer; the third part is the preliminary study on the establishment of human embryonic stem cells. In this paper, the remaining frozen embryo after DSP 3 transplantation was cultured into DSP 5-7 by sequential culture. The formation of blastocyst and the quality of blastocyst were observed, and the relationship between the cell number and quality grading of DSP 3 embryo and the formation of blastocyst and high quality blastocyst were analyzed. To provide theoretical support for blastocyst transplantation. A total of 203 embryos with no cryopreservation value were obtained from 98 patients after DST3 transplantation, and entered the experiment after the patients signed the informed consent form. The embryos were transferred into G2.3, the development of embryos was observed every day until fertilization, and the blastocysts were classified. A total of 51 blastocysts were formed and the blastocyst formation rate was 25.12. There were 14 high-quality blastocysts, accounting for 27.4545 of all blastocysts. The clinical pregnancy rate was 50, which was higher than that in 66 non-blastocyst patients (28.79%). Through analysis, we concluded that there was a significant positive correlation between blastocyst formation rate and blastocyst formation rate. The blastocyst formation rate of 鈪，

本文编号：2032035