金黄地鼠神经管cDNA文库的构建及高温致畸相关基因的筛选

发布时间：2018-06-18 11:05

本文选题：cDNA文库 + 神经管　；参考：《山东大学》2005年博士论文

【摘要】：神经管发生是一个建立中枢神经系统原基的重大胚胎事件,是指从神经板出现到神经沟闭合的胚胎发育过程。在此过程中,只有神经沟及时准确地闭合形成神经管,神经系统才能正常发育,否则将出现神经管畸形。神经管的发生和分化过程吸引了诸多研究者的关注,目的是探讨神经管畸形发生的机制。神经管畸形(Neural tube defects,NTDs)是一类发生率很高的先天畸形,表现为脑脊髓膨出、无脑、露脑等,发生率约为1/1000,是发生率最高的一类先天畸形。目前关于神经管发生和分化的细胞学机制研究很多,但对于其分子机制的研究报道很少。 NTDs可由遗传因素和各种环境因素引起。流行病学调查和动物实验显示,高温是一种最常见且难以避免的物理致畸因素,可导致人及多种动物发生先天畸形,其中以NTDs最为多见。因此,探讨高温致NTDs的机理便成了实验畸形学中的一个研究热点。近年来,人们从细胞、亚细胞和分子水平对高温致畸的机理进行了大量的动物实验和细胞培养研究,观察了高温对细胞增殖、分化、凋亡、粘着、组织诱导和器官发生等方面的影响。研究发现,在神经管形成和分化过程中,某一或某些相关基因的不表达、过表达或低表达都会引发神经管发育异常,造成NTDs。神经管形成是诸多细胞行为按一定顺序发生的结果,涉及到一系列基因的开放和表达。NTDs的发生也并不是单一基因引起的,而是多种基因参与的结果。因此,研究调控神经管正常发育和导致
[Abstract]:The occurrence of neural tube is a major embryonic event of the establishment of the primordium of the central nervous system. It refers to the process of embryonic development from the appearance of the nerve plate to the closure of the nerve groove. In this process, only the nerve trench closes and forms the nerve canal in time and accurately, and the nervous system can develop normally. Otherwise, the neural tube malformation will occur. The occurrence and differentiation of the nerve canal will be found. The process has attracted the attention of many researchers. The purpose is to explore the mechanism of the occurrence of neural tube malformation. Neural tube defects (NTDs) is a kind of congenital malformation with high incidence. It is characterized by encephalocele, no brain, and exposure to brain, and the incidence is about 1/1000. It is a kind of congenital malformation with the highest incidence of birth. There are many studies on the mechanism of differentiation and differentiation, but few studies have been done on its molecular mechanism.
NTDs can be caused by genetic factors and various environmental factors. Epidemiological investigation and animal experiments show that high temperature is one of the most common and difficult physical teratogenicity factors, which can cause congenital malformation of human and many animals, among which NTDs is the most common. Therefore, the study of the mechanism of high temperature NTDs has become one of the research of experimental malformation. In recent years, a large number of animal experiments and cell culture studies have been carried out on the mechanism of high temperature teratogenesis from cellular, subcellular and molecular levels. The effects of high temperature on cell proliferation, differentiation, apoptosis, adhesion, tissue induction and organogenesis are observed. The non expression of related genes, overexpression or low expression can cause the abnormal development of neural tube, and the formation of NTDs. neural tube is the result of a certain sequence of cell behavior. The opening and expression of a series of genes are not caused by a single gene, but the result of a variety of gene participation. Normal development and cause of controlled nerve canal
【学位授予单位】：山东大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R346

【共引文献】