EDS-40玻璃化液冻贮小鼠及人卵母细胞的研究

发布时间：2018-06-20 05:18

本文选题：玻璃化技术 + 玻璃化溶液　；参考：《重庆医科大学》2005年硕士论文

【摘要】：目的:研究 EDS-40 玻璃化溶液冻贮小鼠及人卵母细胞的效果。方法:设计研究 EDS-40 玻璃化溶液。按随机分组原则,分为实验组和对照组,实验组(a)采用自行设计的 EDS-40 玻璃化液,对照组(b)采用国际上公认的效果最好的 EFS-40 玻璃化溶液,对照组(c)采用程序冷冻法。 1、对 EDS-40 溶液行玻璃化测试。 2、鼠卵冻贮:将小鼠卵母细胞按上述原则随机分成 a、b、c 三组, a、b 组在 25°C 室温下,分别加入 EFS-40 和 EDS-40 玻璃化溶液后装管并迅速投入液氮中,c 组用程序冷冻法进行冻存。三组冻贮 2～ 3 个月后复温,用培养液反复洗涤后移入培养孔板培养,观察卵母细胞的成活率。将成活的卵母细胞行体外受精,观察其受精的情况。实验结果用 SPSS11.5 软件进行统计学分析。 3、人卵冻贮:在成功玻璃化冷冻小鼠卵母细胞的基础上,再随机选用人卵母细胞,其冷冻方法、复温方法及统计学处理同动物实验。结果 : 1、EDS-40 玻璃化溶液能达到玻璃化效果。 2、对小鼠卵母细胞两种玻璃化溶液的毒性有显著性差异,EDS-40较 EFS-40 毒性低。小鼠卵母细胞玻璃化冷冻后各组存活率分别为:79.34%、67.94%、56.34%,受精率分别为:79.17%、66.29%、51.14%。 3、人卵母细胞冷冻后 a、b、c 三组各组存活率分别为:53.57 %、66.67%、56.67 %,受精率为 40.00%、77.78%、41.18%。
[Abstract]:Objective: to study the effect of cryopreservation of mouse and human oocytes with EDS-40 vitrification solution. Methods: the glass solution of EDS-40 was designed and studied. According to the principle of random grouping, the experimental group and control group were divided into experimental group and control group. The experimental group was treated with self-designed EDS-40 vitrification solution, while the control group was treated with EFS-40 vitrification solution, which was internationally recognized as the best. (1) vitrification of EDS-40 solution. 2. Storage of mouse eggs. The oocytes were randomly divided into three groups according to the above principles. After adding EFS-40 and EDS-40 vitrified solution respectively, the tube was filled and rapidly put into liquid nitrogen group C for freezing by programmed freezing method. After 2 ~ 3 months of frozen storage, the three groups were incubated by repeated washing of culture medium and transferred into the culture hole plate to observe the survival rate of oocytes. The surviving oocytes were fertilized in vitro to observe their fertilization. The results were statistically analyzed by SPSS 11.5 software. 3. Human oocyte storage: on the basis of successful vitrification of mouse oocytes, human oocytes were selected randomly. The rewarming method and statistical treatment were the same as the animal experiment. Results: 1 the vitrification effect of EDS-40 was achieved. 2. Two kinds of vitrification solutions were applied to mouse oocytes. The toxicity of EDS-40 was lower than that of EFS-40. The survival rate of each group after vitrified freezing of mouse oocytes was 79.34 and 67.94 and 56.34, respectively. The fertilization rate was 79.1766.291.14.3, the survival rate of human oocyte frozen group was 53.57, 66.6756.67, and the fertilization rate was 40.000.77.780.The survival rate of each group was 41.180.The survival rate of each group was 53.57.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：Q813;R321

【共引文献】