人Hedgehog信号通路配体SHH-N端蛋白原核表达及生物学功能分析

发布时间：2018-06-20 14:46

本文选题：Hedgehog + IPMN　；参考：《第二军医大学》2007年硕士论文

【摘要】： 目的:利用SHH-N/pET22b重组大肠杆菌,诱导融合蛋白表达,免疫家兔制备多抗血清。检测胰腺导管内乳头状粘液性肿瘤(IPMN)组织中SHH的表达。方法:SHH-N/pET22b重组大肠杆菌经IPTG诱导表达,Western免疫印迹鉴定。融合蛋白免疫家兔制备多抗血清,免疫组化检测IPMN组织中SHH的表达。结果:成功诱导表达目的蛋白。SHH多抗血清经Western免疫印迹鉴定。免疫组化检测IPMN标本呈阳性表达。结论:重组质粒SHH-N/pET22b成功表达目的蛋白;并制备了SHH多抗血清,为研究Hedgehog信号通路在胰腺癌中的发病机制提供了有效工具。
[Abstract]:Aim: to induce the expression of fusion protein by Recombinant Escherichia coli SHH-N / pET22b and immunize rabbits to prepare polyclonal antiserum. The expression of SHH in pancreatic intraductal papillary mucinous tumor (IPMN) was detected. Methods the recombinant Escherichia coli was identified by Western blot after IPTG induced expression. The fusion protein was used to immunize rabbits to prepare polyantiserum and to detect the expression of SHH in IPMN tissue by immunohistochemistry. Results: the target protein. SHH polyantibodies were successfully induced and identified by Western blot. The positive expression of IPMN was detected by immunohistochemistry. Conclusion: the recombinant plasmid SHH-N / pET22b successfully expressed the target protein and prepared SHH polyantiserum, which provides an effective tool for studying the pathogenesis of Hedgehog signaling pathway in pancreatic cancer.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R341;R735.9

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