人肠道病毒71型高致病性毒株及其体外适应株cDNA感染性克隆的构建

发布时间：2018-06-20 18:39

本文选题：肠道病毒型 + 感染性克隆　；参考：《病毒学报》2014年06期

【摘要】：高致病性EV71毒株的感染易伴发神经系统症状,是导致手足口病死亡病例的主要原因,但目前临床上仍缺乏防治EV71感染的特效药物及安全有效的疫苗。为进一步明确EV71的致病机制,本项工作以高致病性的EV71-HP临床分离株及其经体外细胞培养适应的EV71-CCA毒株的基因组RNA为模板,应用反向遗传学方法构建了二者的感染性克隆。研究表明HP株在细胞中的增殖速度明显快于CCA株。两株基于感染性克隆的拯救病毒分别保持了与各自母本株基因组序列的一致性,同时也保留了母本株增殖表型的差异,包括病毒的增殖速度以及病毒的温度敏感性。本工作将促进对EV71的致病机制以及对EV71疫苗的研究。
[Abstract]:The infection of highly pathogenic EV71 strain is easy to be accompanied by nervous system symptoms, which is the main cause of death of hand, foot and mouth disease. However, there is still a lack of effective drugs to prevent and treat EV71 infection and a safe and effective vaccine. In order to further clarify the pathogenic mechanism of EV71, the highly pathogenic EV71-HP clinical isolates and the EV71-CCA strain adapted by cell culture in vitro were used as templates to construct the infectious clones of EV71-CCA by reverse genetics. The results showed that the proliferation rate of HP strain was significantly faster than that of CCA strain. The two rescue viruses based on infectious clones were consistent with the genomic sequences of their mother strains, and retained the differences in phenotypic proliferation of the female strains, including the speed of virus proliferation and the temperature sensitivity of the virus. This work will promote the pathogenesis of EV 71 and the study of EV 71 vaccine.
【作者单位】：中国医学科学院医药生物技术研究所;首都医科大学附属北京地坛医院;中国疾病预防控制中心病毒病预防控制所;
【基金】：十二五“新药创制”科技重大专项基金(2012ZX09301-002-004) 十二五“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项基金(2013ZX10004601-002) 国家自然科学基金(81401675)
【分类号】：R373.2

【共引文献】