胎盘源间充质干细胞对T淋巴细胞体外增殖和细胞因子分泌的影响

发布时间：2018-06-26 09:03

本文选题：间充质干细胞 + 胎盘　；参考：《苏州大学》2007年硕士论文

【摘要】： 目的:间充质干细胞(mesenchymal stem cells,MSCs)具有自我更新和多向分化的潜能,被认为在移植治疗、基因治疗、细胞治疗、组织工程等领域具有广阔的临床应用前景。而MSCs的来源以及MSCs的免疫调节机制是亟需解决的两大问题。本实验的目的旨在从人胎盘组织中分离培养获得间充质干细胞,并进一步研究其对T淋巴细胞体外增殖和细胞因子分泌的影响。方法:首先从胎盘组织中分离培养胎盘间充质干细胞(PMSCs),在体外观测其形态,并通过细胞表面抗原表达、分化潜能等特征进行鉴定;然后将体外分离培养扩增的PMSCs,按照不同比例加入双向混合淋巴细胞培养体系(mixed lymphocyte reaction, MLR)中,共同培养3d后,用ELISA法检测各组MLC上清中细胞因子IL-2、IL-4与IFN-γ的含量以及共同培养6d后,测定T淋巴细胞的增殖。结果:1.从人胎盘组织中分离培养获得间充质干细胞,具有与骨髓间充质干细胞(BMSCs)极为相似的细胞形态及细胞表面标志,表达CD29、CD44和CD105,不表达CD34、CD45,、CD106和HLA-DR。并且还具有与BMSCs相似的跨胚层分化能力,能在一定条件下被诱导分化出神经元样细胞。2.PMSCs与同种异体混合淋巴细胞共同培养后,能抑制T淋巴细胞的体外增殖和影响细胞因子IL-2、IL-4与IFN-γ的分泌。结论:胎盘组织是MSCs的有效来源,PMSCs具有与BMSCs相似的生物学特性及免疫调节机制。它为间充质干细胞的研究提供了又一重要的细胞来源。
[Abstract]:Objective: mesenchymal stem cells (MSCs) have the potential of self-renewal and multi-differentiation. They are widely used in transplantation therapy, gene therapy, cell therapy, tissue engineering and so on. The origin of MSCs and the immunomodulation mechanism of MSCs are two problems that need to be solved. The aim of this study was to isolate and culture mesenchymal stem cells from human placenta and to investigate the effects of mesenchymal stem cells on T lymphocyte proliferation and cytokine secretion in vitro. Methods: placental mesenchymal stem cells (PMSCs) were isolated and cultured from placenta tissue. The morphology of PMSCs was observed in vitro. After 3 days of co-culture, the levels of cytokines IL-2, IL-4 and IFN- 纬 in the supernatant of MLC were detected by Elisa and co-cultured for 6 days. The proliferation of T lymphocytes was measured. The result is 1: 1. Mesenchymal stem cells (MSCs) isolated from human placental tissue have similar cell morphology and cell surface markers to bone marrow mesenchymal stem cells (BMSCs). They express CD29, CD44 and CD105, and do not express CD34, CD45, CD106 and HLA-DR. It also has the ability of transdermal differentiation similar to that of BMSCs, and can be induced to differentiate into neuron-like cells. 2. PMSCs can be co-cultured with allogenic mixed lymphocytes. It can inhibit the proliferation of T lymphocytes in vitro and affect the secretion of cytokines IL-4 and IFN- 纬. Conclusion: placental tissue is an effective source of MSCs, PMSCs have similar biological characteristics and immunomodulatory mechanism as BMSCs. It provides another important cell source for the study of mesenchymal stem cells.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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