人内皮抑素基因的克

发布时间：2018-06-26 18:03

本文选题：子宫内膜异位症 + 人内皮抑素　；参考：《第一军医大学》2005年硕士论文

【摘要】：研究背景子宫内膜异位症(Endometriosis,简称内异症)是一种育龄妇女的常见病,发病率逐年上升,已达10%-15%,35%-50%的不孕妇女患有该病,由其引起的痛经、不孕、性交疼痛等症状严重影响着妇女的生活质量。内异症真正的病因和发病机制尚不明确。现有的治疗方法局限于激素类药物、手术或手术联合药物等,虽对缓解症状,提高妊娠率有一定帮助,但疗效有限,副作用大,复发率高。近年来的研究表明,脱落的子宫内膜附着在腹膜或其它部位时,异位内膜本身及其周围组织新的血供的建立和维持,是异位内膜种植存活和子宫内膜异位症发生的基本条件。血管生成机制已成为内异症公认的重要发病机制之一。因此,从血管生成理论出发,寻找内异症新的治疗途径,降低内异症复发和减少毒副作用已成为临床的迫切需要。内皮抑素作为目前已知最强的内源性血管形成抑制因子,在肿瘤的治疗方面已有大量研究及应用,但在内异症的治疗研究领域鲜有报道。目的本研究旨在通过分子生物学技术,从胎肝组织克隆人内皮抑素基因,在大肠杆菌中表达,纯化后得到有生物学活性的重组蛋白质产物,为后续的动物实验奠定基础。方法与结果 1.采用RT-PCR技术从胎肝中克隆人内皮抑素基因全序列,构建人内皮抑素
[Abstract]:Background Endometriosisis (Endometriosism) is a common disease among women of childbearing age. The incidence of endometriosis has increased year by year. Symptoms such as dysmenorrhea, infertility, and pain in sexual intercourse seriously affect women's quality of life. The true etiology and pathogenesis of endometriosis are unclear. The existing treatment methods are limited to hormone drugs, surgical or surgical drugs, although to alleviate symptoms and improve pregnancy rate, but the curative effect is limited, side effects are large, recurrence rate is high. Recent studies have shown that the establishment and maintenance of new blood supply of ectopic endometrium and its surrounding tissues are the basic conditions for the survival of ectopic endometrium and the occurrence of endometriosis when the abscission endometrium is attached to the peritoneum or other parts. Angiogenesis has become one of the important pathogenesis of endometriosis. Therefore, based on the angiogenesis theory, it has become an urgent need to find a new way to treat endometriosis, to reduce the recurrence of endometriosis and to reduce the side effects. Endostatin, as the strongest known endogenous angiogenesis inhibitor, has been widely studied and applied in the treatment of cancer. However, there are few reports in the field of treatment of endostatin. Objective to clone human endostatin gene from fetal liver tissue and express it in Escherichia coli by molecular biology. After purification, the recombinant protein with biological activity was obtained, which laid a foundation for further animal experiments. Methods and results 1. Human endostatin gene was cloned from fetal liver by RT-PCR to construct human endostatin.
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346

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