SARS冠状病毒Mc区的克隆表达及表达蛋白抗原活性研究

发布时间：2018-06-27 20:32

本文选题：SARS冠状病毒 + M蛋白Mc区　；参考：《第一军医大学》2005年硕士论文

【摘要】：一、研究背景和目的 SARS冠状病毒(SARS-CoV)是一种新现的病原微生物,属于巢状病毒目(Order:Nidovirales)、冠状病毒科(Family:Coronaviridae)、冠状病毒属(Genus:Coronavirus),基因组为单链正RNA。SARS-CoV可引起严重急性呼吸综合征(Severe Acute Respiratory Syndrome, SARS),传染性强,死亡率高。首发病例于2002年11月出现在中国广东,疫情很快波及全球。目前,SARS暂时得到控制,但在SARS防治中许多关键问题尚待解决,如SARS-CoV入侵细胞及致病机制、特异性抗病毒药物、快速诊断试剂和疫苗的研制等。研究表明,冠状病毒S蛋白(棘突蛋白)和M蛋白(膜蛋白)具有免疫原性,能够诱导中和抗体产生。M蛋白(membrane protein)是最丰富的结构蛋白,它跨膜双分子层3次,短氨基端在膜外,长羧基端在膜内,由121个氨基酸组成,被认为是与核蛋白体作用的区域。本项研究分析了82株SARS-CoV M基因的变异情况,发现氨基酸变异主要发生在膜外和跨膜区域,膜内区域变异极少。对SARS-CoV M蛋白的B细胞抗原表位进行预测,结果表明M蛋白的B细胞抗原表位85%以上都位于M蛋白膜内区(Mc蛋白)。另有研究者预测,M蛋白所含T细胞激活表位主要集中在第81-220位氨基酸之间,占84.7%,即主要集中在Mc蛋白区域内。所以,就抗原活性而言,Mc蛋白应是M蛋白的代表区段,而且Mc蛋白的疏水性小于M蛋白的疏水性,更易于大量体外表达。因此,本项研究将靶蛋白锁定
[Abstract]:Background and objective SARS Coronavirus (SARS-CoV) is a new pathogenic microorganism. It belongs to order: Nidovirales, Family: Coronaviridae, Genus: Coronavirus. The genome is single-stranded positive RNA.SARS-CoV can cause severe Acute Respiratory Syndrome (SARS). The first case occurred in November 2002 in Guangdong, China, the epidemic quickly spread to the world. At present, SARS has been temporarily controlled, but many key problems remain to be solved in the prevention and treatment of SARS, such as SARS-CoV invasion cells and pathogenesis, specific antiviral drugs, rapid diagnostic reagent and vaccine development, etc. The results show that coronavirus S protein (spinous process protein) and M protein (membrane protein) have immunogenicity, and can induce neutralizing antibody to produce. M protein (membrane protein) is the most abundant structural protein. The long carboxyl terminal, composed of 121 amino acids, is believed to interact with ribosomes. In this study, the variation of 82 SARS-CoV M genes was analyzed. It was found that the amino acid variation mainly occurred in the extracellular and transmembrane regions, and there was very little variation in the intramembrane region. The B cell epitopes of SARS-CoV M protein were predicted. The results showed that more than 85% of B cell epitopes of SARS-CoV M protein were located in the intramembrane region of M protein (Mc protein). Other researchers predicted that the T cell activation epitopes contained in M protein were mainly located between the 81-220 amino acids, accounting for 84.7%, that is, mainly in the Mc protein region. Therefore, in terms of antigen activity, Mc protein should be the representative region of M protein, and the hydrophobicity of Mc protein is less than that of M protein, and Mc protein is more easily expressed in vitro. Therefore, this study locks the target protein
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R373

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