中波紫外线对离体巴马香猪皮肤生物特性影响的研究

发布时间：2018-06-29 12:53

本文选题：UVB + 皮肤移植　；参考：《西南大学》2007年硕士论文

【摘要】： 背景异体和异种皮肤是临床上替代自体皮肤治疗大面积烧伤的主要敷料，但异体(异种)皮肤抗原性强，特别是新鲜皮肤，还带有大量的菌污染，皮肤移植后的强烈免疫排斥反应和菌污染往往会导致皮肤过早的坏死。中波紫外线(UVB)，波长280—320nm，它可降低免疫原性，可应用于器官移植的前处理，降低受者对移植物的排斥反应和移植物的抗宿主反应，但UVB是否有灭菌作用，仍未有报道。因此，紫外照射是降低移植皮肤的抗原性、延长移植皮肤存活提供简单、快捷的途径。但是将UVB降低免疫原性应用于皮肤移植的研究缺乏系统的研究，且多大剂量可以达到最理想的效果仍不清楚。研究目的和意义本课题以不同剂量UVB照射离体巴马香猪皮肤为实验材料，研究不同剂量UVB对皮肤的免疫原性、带菌量、活力等指标的影响，旨在寻找可以降低免疫原性、带菌量少、活力较高的UVB照射剂量，为延长移植皮肤存活奠定理论基础。方法与结果 1．免疫原性检测 1．1淋巴细胞增殖实验 UVB以剂量依赖的方式抑制皮肤的免疫原性。30J／cm~2UVB照射时，有轻微抑制，抑制率为14.3％；60J／cm~2UVB照射时，抑制率为42.9％；360J／cm~2UVB照射时，达到最大抑制率，抑制率为99.9％。 1．2 ATP酶染色郎格汉细胞(Langerhans cell，LC) 新鲜皮肤LC ATP酶染色呈棕黑色，树突状；UVB处理后，LC树突减少，甚至消失。UVB处理后LC数量较新鲜皮有显著下降，并且随照射剂量的增加LC数量逐渐下降。UVB处理后皮肤的免疫原性与皮肤中LC数量呈正相关。 2．带菌量检测 UVB具有抑菌作用。随着UVB剂量的增大，皮肤中的带菌量减少，UVB达到本研究中最大剂量180J／cm~2时，皮肤中仍有带菌量。 3．UVB对皮肤的损伤作用用MTT法检测UVB预处理的皮肤活性实验表明，UVB剂量在0—90J／cm~2范围内，皮肤的活力随剂量的增大而减小，当UVB剂量增加到90—360J／cm~2，皮肤的活力又先升后降。结论 UVB具有抑菌作用，可以降低皮肤的免疫原性，但对皮肤有损伤作用；UVB以剂量依赖的方式抑制皮肤的免疫原性，且皮肤的免疫原性与UVB照射后皮肤中LC数量呈正相关；UVB剂量为30-60J／cm~2时，离体皮肤免疫原性低，皮肤损伤小，且带菌量低，可作为异体(异种)皮肤移植的实验基础数据。
[Abstract]:Background Allogeneic skin and xenogeneic skin are the main dressing to replace autologous skin in treating large area burn, but allogeneic skin has strong antigenicity, especially fresh skin. There is also a large number of bacterial contamination, skin transplantation after strong immune rejection and bacterial contamination will often lead to premature skin necrosis. Ultraviolet B (UVB), wavelength 280-320nm. it can reduce immunogenicity, can be used in the preprocessing of organ transplantation, reduce the graft rejection and graft anti-host reaction, but whether UVB has sterilizing effect has not been reported. Therefore, external violet irradiation is a simple and fast way to reduce the antigenicity of transplanted skin and prolong the survival of transplanted skin. However, the study of reducing immunogenicity of UVB in skin transplantation is lack of systematic research, and it is not clear what dose of UVB can achieve the best effect. Objective and significance to study the effects of different doses of UVB on the immunogenicity, bacterial count and vitality of the skin of Bama Xiang pig irradiated with different doses of UVB as experimental materials, and to study the effects of different doses of UVB on the immunogenicity of skin, the amount of bacteria carried and the activity of the skin. The aim of this study was to find a dose of UVB that could reduce immunogenicity, carry less bacteria and have higher activity, and lay a theoretical foundation for prolonging the survival of transplanted skin. Methods and results 1.Immunogenicity test 1.1 lymphocyte proliferation test UVB Inhibition of skin immunogenicity in a dose-dependent manner. 30 J / cm ~ (2) UVB irradiation, There was slight inhibition, the inhibition rate was 14.3J / cm ~ (-2) UVB irradiation, and the inhibition rate was 42.9% ~ 360J / cm ~ (-2) UVB irradiation, which reached the maximum inhibition rate. The inhibitory rate was 99.9%. 1.2 ATP enzyme staining was found in the fresh skin of Langerhans cellulocytes. LC ATPase staining was brown and black, and the dendritic dendrites decreased after UVB treatment. The number of LC after treatment with UVB was significantly lower than that of fresh skin. The immunogenicity of skin treated with UVB was positively correlated with the number of LC in skin. 2. UVB has bacteriostatic effect. With the increase of UVB dose, the amount of bacteria carried in the skin decreased to the maximum dose of 180 J / cm ~ 2 in this study. 3. The damage effect of UVB on skin. 3. The effect of UVB on skin injury. 3. The skin activity of UVB pretreatment was detected by MTT method. 3. The dose of UVB was measured by MTT assay. In the 0-90 J / cm2 range, The skin activity decreased with the increase of UVB dose. When the UVB dose increased to 90-360 J / cm ~ (2), the skin activity increased first and then decreased. Conclusion UVB can inhibit the immunogenicity of skin, but it can inhibit the immunogenicity of skin in a dose-dependent manner. The immunogenicity of the skin was positively correlated with the amount of LC in the skin after UVB irradiation. The dose of UVB was 30-60 J / cm ~ 2. The immunogenicity of the skin in vitro was low, the skin damage was small, and the amount of bacteria was low, which could be used as the experimental data of xenogeneic skin transplantation.
【学位授予单位】：西南大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R622;R312

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