人呼吸道上皮细胞Nod2受体的表达与调控-Nods受体在天然免疫中作用的初探

发布时间：2018-07-01 13:08

本文选题：天然免疫 + 呼吸道上皮细胞　；参考：《四川大学》2006年硕士论文

【摘要】：天然免疫系统近年来颇受关注，它是生物体赖以生存的最基本条件，是抵御微生物入侵的第一道屏障，是生物体在长期种系发生和进化过程中逐渐形成的，对各种病原微生物都具有抗感染的作用。与获得性免疫系统一起组成了人体抵御外界的两大重要防御机制。天然免疫识别的是微生物生存所必需的，相对保守的，并且宿主不具备的病原体相关模式分子(pathogen-associated molecular patterns，PAMP)，包括革兰阴性菌的脂多糖(LPS)、革兰阳性菌的磷壁酸、病毒的双链RNA、细菌的非甲基化CpGDNA、细菌的蛋白特征分子甲酰甲硫氨酰肽等。而天然免疫系统的受体则称为模式分子识别受体(pattern recognition receptors，PRRs)。识别受体是在种系发生中产生，是长期自然选择的结果。目前研究较多的两种主要的受体分别为Toll受体与Nods蛋白受体。经研究表明Nods蛋白受体，主要是Nod1和Nod2，分布在肠道等上皮细胞胞浆中，，能识别细菌胞壁肽聚糖成分，能被细胞因子及侵袭性细菌上调表达，激活NF-κB信号途径，启动机体的免疫应答和炎症反应。本项研究的目的是检测呼吸道上皮细胞和膀胱上皮细胞中有无Nod2的表达，并进一步探讨其表达的调控因素以及其胞内信号转导通路。
[Abstract]:The innate immune system has attracted much attention in recent years. It is the most basic condition for the survival of organisms, the first barrier against the invasion of microorganisms, and the gradual formation of organisms in the course of long-term phylogeny and evolution. It has anti-infection effect to various pathogenic microorganisms. Together with the acquired immune system, the body has two important defense mechanisms against the outside world. Innate immunity recognizes pathogen-associated molecular patterns associated with PAMP, which are necessary for microbial survival and are not available to the host, including lipopolysaccharide (LPS) of gram-negative bacteria, and phosphoric acid from gram-positive bacteria. Double stranded RNAs of viruses, demethylated CpGDNAs of bacteria, protein characteristic molecules of bacteria, formylmethionine peptide, etc. The receptors in the innate immune system are called pattern molecule recognition receptors (pattern recognition receptors). Recognition receptors are the result of long-term natural selection. The two main receptors are Toll receptor and Nods protein receptor. The results showed that Nods protein receptors, mainly Nod1 and Nod2, distributed in the cytoplasm of intestinal epithelial cells, recognized peptidoglycan components of bacterial wall, up-regulated expression by cytokines and invasive bacteria, and activated NF- 魏 B signaling pathway. Activate the body's immune response and inflammatory response. The aim of this study was to detect the expression of Nod2 in respiratory epithelial cells and bladder epithelial cells, and to further investigate the regulatory factors and intracellular signal transduction pathways of Nod2 expression.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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