椎间盘源性下腰痛动物模型的建立及行为学观察

发布时间：2018-07-03 10:50

本文选题：椎间盘源性下腰痛 + 动物模型　；参考：《中国人民解放军军医进修学院》2006年博士论文

【摘要】：目的:建立椎间盘源性下腰痛的动物模型,探讨其发病机制,观察椎间盘源性下腰痛动物的行为学表现。方法和研究内容:实验用雄性SD大鼠共96只,随机分为以下三组:对照组(假手术组)、手术一组、手术二组。全身麻醉后取仰卧位,采用经腹腔正中入路,暴露L6-7椎间盘。于椎间盘前侧正中点以直径0.2mm的特制针头刺入L6-7椎间盘至后侧外层纤维环下方,深度控制为3mm(手术一组)。或在与第一针道各交叉呈30度角左右分别穿刺至后外侧纤维环下方(手术二组)。各时相点分别观察自发性行为学表现。取L6-7椎间盘,观察病理学改变,采用免疫组织化学方法检测椎间盘内CD34、IL-1β和COX-2的表达情况。主要结果: 1.正常椎间盘髓核内可见纤维组织样细胞,交织成网状。术后髓核内纤维组织样细胞透明样变性,与胶冻状物交织在一起,内层纤维软骨细胞变性。 2.手术椎间盘内可见由前方至后方的裂隙,后侧的外层纤维环未破裂,该表现符合椎间盘源性下腰痛的病理表现。 3.术后可在内层纤维环内层见到散在的、染色较强的CD34阳性细胞,提示有血管内皮细胞形成。 4.术后内层纤维环可见多处、散在棕染IL-1β阳性细胞,呈环形分布,外层纤维环很少或无阳性细胞。 5.术后髓核内多数、散在纤维组织样细胞胞浆棕染呈COX-2阳性,少数内层纤维环纤维软骨细胞可见稀疏、淡染阳性着色。 6.术后手术二组实验大鼠的静止不动时间延长、运动时间减少,同假手术组相比,有统计学意义。
[Abstract]:Objective: to establish an animal model of discogenic low back pain, to investigate its pathogenesis and to observe the behavior of low back pain caused by intervertebral disc. Methods: 96 male Sprague-Dawley rats were randomly divided into three groups: control group (sham operation group), operation group and operation group. After general anesthesia, supine position was taken, and L-6-7 disc was exposed via the median peritoneal approach. The L6-7 disc was punctured with a special needle with diameter 0.2mm at the anterior and middle point of the disc to the posterior outer fibrous ring. The depth of the disc was controlled as 3mm (surgical group). Or at 30 degrees angle with the first needle, puncture to the posterolateral fibrous ring respectively (operation group 2). Spontaneous behavior was observed at each time point. The expressions of IL-1 尾 and COX-2 in intervertebral discs were detected by immunohistochemical method. Main results: 1. Fibrous tissue-like cells can be seen in the nucleus pulposus of normal intervertebral disc, intertwined into reticular form. After operation, fibroid-like cells in the nucleus pulposus showed hyaline denaturation, intertwined with gelatinous material, and denaturation of fibrochondrocytes in the inner layer of the nucleus pulposus. 2. 2. A fissure was seen in the intervertebral disc from the front to the rear, and the outer fibrous ring of the posterior side was not ruptured, which was consistent with the pathological manifestation of low back pain caused by intervertebral disc. Scattered and strongly stained CD34 positive cells could be seen in the inner layer of the inner fibrous ring after operation, indicating the formation of vascular endothelial cells. 4. There were many spots in the inner fibrous ring, scattered in the brown stained IL-1 尾 positive cells, distributed in a circular pattern, and there were few or no positive cells in the outer fibrous ring. After operation, most of the nucleus pulposus were stained with COX-2 positive in cytoplasm of scattered fibrous tissue like cells, a few fibrochondrocytes in the inner layer were sparse and light staining positive staining was 6. 6%. The time of immobility was prolonged and the time of exercise was decreased in the two groups after operation, which was statistically significant compared with the sham operation group.
【学位授予单位】：中国人民解放军军医进修学院
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R-332;R681.5

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