磷酸化p38 MAPK在小鼠卵泡发育中的表达

发布时间：2018-07-03 19:31

本文选题：p-p + MAPK　；参考：《中国妇幼保健》2014年23期

【摘要】：目的:通过检测磷酸化p38 MAPK(p-p38MAPK)在小鼠卵泡发育过程中的表达,为研究丝裂原活化蛋白激酶(MAPKs)信号通路在卵泡生长、发育及成熟过程中的作用提供理论基础。方法:随机选取健康成年小鼠60只,按雌雄2∶1的比例合笼繁殖新生小鼠。取3、5、7、11及21天雌性健康小鼠(各30只)的卵巢组织,采用免疫组化SP法检测不同年龄段小鼠卵巢组织中p-p38MAPK蛋白的表达水平。结果:p-p38MAPK蛋白在各年龄段小鼠不同级别卵泡的卵母细胞胞浆中均有表达,在始基卵泡中表达最强,与各级卵泡相比,差异均有统计学意义(P0.05);初级卵泡与次级卵泡比较,差异无统计学意义(P0.05);初级卵泡、次级卵泡与窦状卵泡相比,差异均有统计学意义(P0.05)。该蛋白在各级卵泡颗粒细胞中也均表达,始基卵泡与各级卵泡相比,差异均有统计学意义(P0.05);初级卵泡与次级卵泡相比差异有统计学意义(P0.05),而与窦状卵泡相比差异无统计学意义(P0.05);在次级卵泡中表达最强,与各级卵泡相比差异均有统计学意义(P0.05)。不同年龄段小鼠卵巢中,21天小鼠卵母细胞p-p38MAPK蛋白表达强度分别与3天和7天小鼠进行比较,差异均有统计学意义(P0.05);3、5、7及11天各组相比,差异均无统计学意义(P0.05)。各年龄组颗粒细胞p-p38MAPK蛋白表达强度相比,差异均有统计学意义(P0.05)。结论:p-p38MAPK蛋白在小鼠卵泡发育过程中具有时空特异性,p-p38MAPK通路可能在卵泡发育过程中起重要的调控作用。
[Abstract]:Objective: to provide a theoretical basis for the study of the role of mitogen activated protein kinase (MAPKs) signaling pathway in the process of follicle growth, development and maturation by detecting the expression of phosphorylated p38 MAPK (p-p38MAPK) in the follicular development of mice. Methods: 60 healthy adult mice were randomly selected to reproduce the newborn babies according to the proportion of male and female 2 to 1. The expression level of p-p38MAPK protein in ovarian tissue of mice of different ages was detected by immunohistochemical SP method. Results: the expression of p-p38MAPK protein was expressed in the cytoplasm of different levels of the oocytes of mice of different ages, and the expression of the p-p38MAPK protein was the strongest in the basal follicles. The difference between the primary follicles and the secondary follicles was statistically significant (P0.05). The primary follicle and the secondary follicle were not statistically significant (P0.05). The primary follicle and secondary follicle were significantly different from the sinus follicles (P0.05). The protein was also expressed in the follicle granulosa cells at all levels, and the basal follicles were different from the follicles at all levels. Statistical significance (P0.05); the primary follicle had a statistically significant difference compared with the secondary follicle (P0.05), but had no statistical significance compared with the sinus follicle (P0.05); the strongest expression in the secondary follicle was statistically significant (P0.05). In mice of different age groups, the 21 day mouse oocyte p-p38MAPK eggs The white expression intensity was compared with the 3 day and 7 day mice, the difference was statistically significant (P0.05), and there was no significant difference between 3,5,7 and 11 days (P0.05). The difference of p-p38MAPK protein expression intensity in all age groups was statistically significant (P0.05). Conclusion: p-p38MAPK protein is in the process of mouse follicle development. Sometimes the p-p38MAPK pathway may play an important role in the regulation of follicular development.
【作者单位】：华中科技大学同济医学院附属同济医院妇产科;
【基金】：国家自然科学基金〔81370469〕科技部国家国际科技合作专项项目基金〔2013DFA31400〕
【分类号】：R321

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