石杉碱甲快速检测酶联免疫试剂盒的制备

发布时间：2018-07-05 16:25

  本文选题：石杉碱甲 + 单克隆抗体　； 参考：《中国免疫学杂志》2014年01期

【摘要】：目的:制备并鉴定石杉碱甲单克隆抗体,研制石杉碱甲间接竞争酶联免疫试剂盒。方法:以石杉碱甲人工抗原免疫BALB/c小鼠获得单克隆抗体,采用酶联免疫吸附法制备石杉碱甲试剂盒,对试剂盒各项指标进行评价。结果:试剂盒工作范围为5~2 500 ng/ml,检测限为6.242 ng/ml,与多种生物碱均无交叉反应,试剂盒可在4℃或-20℃下稳定贮存半年以上,石杉碱甲片回收率在94.2%~108.6%之间,变异系数在2.3%~4.8%之间。结论:石杉碱甲ELISA试剂盒快速、灵敏、稳定,可用于石杉碱甲的含量测定。
[Abstract]:Aim: to prepare and identify monoclonal antibody against Huperzine A and to develop an indirect competitive enzyme linked immunosorbent kit (Elisa) for Huperzine A. Methods: monoclonal antibodies were obtained from BALB / c mice immunized with Huperzine A artificial antigen, and Huperzine A kit was prepared by enzyme-linked immunosorbent assay (Elisa). Results: the working range of the kit was 5 ~ 2 500 ng / ml, the detection limit was 6.242 ng / ml, and there was no cross reaction with a variety of alkaloids. The kit could be stably stored at 4 鈩，

本文编号：2100893