表皮生长因子及其受体在食蟹猴出生后睾丸发育过程中的表达研究

发布时间：2018-07-10 09:38

本文选题：表皮生长因子 + 表皮生长因子受体　；参考：《广西医科大学》2005年硕士论文

【摘要】：目的观察表皮生长因子(EGF)及其受体(EGFR)在出生后食蟹猴(MacacaFascicularis)睾丸发育成熟过程中的表达特征,探讨两者在睾丸发育成熟中的作用。材料与方法32只雄性食蟹猴根据睾丸曲细精管发育程度分为5个发育阶段:新生猴期5 只、童猴前期5 只、童猴后期7 只、青春猴期7 只、成年猴期8 只。获取的睾丸组织均用Bouin’s 溶液固定4-12 小时,常规石蜡包埋,切片厚度6μМ,贴附在多聚赖氨酸处理过的载玻片上。切片脱蜡后常规HE染色。用Eli VisionTM免疫组织化学技术检测食蟹猴从新生猴期至成年猴期不同发育阶段睾丸EGF、EGFR 的表达。用病理图像分析系统计算各发育阶段睾丸的阳性细胞率,用SPSS10.0 统计软件包对睾丸各发育阶段的阳性细胞率进行单因素的方差分析。结果32 只食蟹猴睾丸均表达EGF 和EGFR。EGF 主要表达于食蟹猴曲细精管内的精原细胞及一些间质细胞的胞浆中,偶见表达于支持细胞胞浆。各发育阶段睾丸精原细胞EGF 阳性率比较在统计学上有显著性差异P0.01,其阳性细胞率在新生猴期最低,童猴后期最高,随后呈下降趋势。EGFR 表达于食蟹猴睾丸的间质细胞胞浆,曲细精管内细胞未见阳性染色。各发育阶段睾丸间质细胞EGFR 阳性率比较在统计学上有显著性差异P0.01,其阳性细胞率在新生猴期最大,童猴前期最低,以后又随睾丸的发育而增加,在成年猴期达到第二高峰。结论EGF 及其受体在在睾丸发育过程中的不同表达,提示EGF 及其受体对睾丸的形态发育及精子发生起调节作用。
[Abstract]:Objective to investigate the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in testis of Macaca fascicularis (Macaca fascicularis) and to explore the role of EGF and EGFR in testicular maturation. Materials and methods 32 male crab-eating monkeys were divided into five stages according to the development degree of testicular seminiferous tubules: 5 in newborn monkey, 5 in prophase, 7 in later stage, 7 in adolescence and 8 in adult monkey. The obtained testis were immobilized in Bouinine solution for 4-12 hours, embedded in conventional paraffin wax, with a thickness of 6 渭 m and attached to the polylysine treated slides. Section dewaxing routine HE staining. Eli VisionTM immunohistochemical technique was used to detect the expression of EGFR in testis of crab eating monkey from newborn monkey to adult monkey. The positive cell rate of testis was calculated by pathological image analysis system, and the single factor analysis of variance was carried out by SPSS 10.0 software package. Results EGF and EGFR.EGF were mainly expressed in spermatogonia and some interstitial cells of seminiferous tubule in 32 monkey testis, and occasionally in the cytoplasm of Sertoli cells. The positive rate of EGF in testicular spermatogonia was significantly different in all stages of development (P0.01). The positive rate of EGF was the lowest in the newborn monkey stage and the highest in the later stage of childhood monkey, and then decreased. EGFR was expressed in the cytoplasm of the mesenchymal cells of the testis of the crab-eating monkey. No positive staining was found in the cells in the seminiferous tubules. The positive rate of EGFR in Leydig cells of testis in all developmental stages was significantly different (P0.01). The positive rate of EGFR was the highest in the newborn monkey stage and the lowest in the early stage of the juvenile monkey, and then increased with the development of the testis, and reached the second peak in the adult monkey stage. Conclusion different expressions of EGF and its receptors during testicular development suggest that EGF and its receptors regulate testicular morphology and spermatogenesis.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R-332

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