甲状腺激素制作SD雄性大鼠肾阴虚证动物模型的研究

发布时间：2018-07-10 14:09

本文选题：肾阴虚 + SD大鼠　；参考：《广州中医药大学》2006年硕士论文

【摘要】：一、目的: 本课题的目的在于探索甲状腺激素导致肾阴虚证动物模型的规范造模方法,同时提供一个初步的肾阴虚证动物模型评价标准,为中医证候动物模型规范化研究提供参考。二、方法: 1.理论研究:肾阴虚证动物模型评价指标选择 1.1 通过查阅资料总结出临床肾阴虚证诊断标准及客观指标研究成果,包括症状体征和客观评价指标。 1.2 根据临床诊断标准和评价指标初步拟定肾阴虚证动物模型评价指标。 2.实验研究(分以下四步进行) 2.1 实验一:初步筛选甲状腺素导致肾阴虚证大鼠模型的规范化造模方法。本实验选择SPF级雄性SD大鼠,根据文献记载的最常用剂量设计出三个剂量:25mg/kgbw、12.5mg/kgbw、6.25mg/kgbw造模,分别在造模后第14天、21天、28天连续采血,观察各剂量组动物血清皮质醇(Cort)、睾酮(T)、雌二醇(E_2)、甲状腺素T_3和T_4、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)等各项指标随剂量、时间的变化,与空白对照组作对照,确定用药剂量和给药时间,筛选出较为规范的造模方法。 2.2 实验二:初步筛选甲状腺素加利血平制作肾阴虚证大鼠模型的方法。本实验选择SPF级雄性SD大鼠,根据文献记载的最常用剂量设计出三个剂量组:甲状腺素37.5mg/kgbw、利血平0.25mg/kgbw;甲状腺素75mg/kgbw、利血平0.5mg/kgbw;甲状腺素150mg/kgbw、利血平1.0mg/kgbw造模,分别在造模后第6天、10天、14天连续采血,观察各剂量组动物血清皮质醇(Cort)、睾酮(T)、雌二醇(E_2)、甲状腺素T_3和T_4、环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)等各项指标随剂量、时间的变化,与空白对照组作对照,确定用药剂量和给药时间,筛选出较为规范的造模方法。 2.3 实验三:甲状腺激素造模法对雄性SD大鼠症状及其它指标的影响按实验一实验二筛选出的造模方法造模,在造模过程中对动物进行一般情况的观察,并进行耐力耐寒试验、抓取激惹试验、隔离攻击试验、旷场试验、母鼠怀孕率等试验,与空白对照组作对照。通过对症状的科学观察,补充前面两个实验客观指标检测的不足,并进一步完善肾阴虚证动物模型症状诊断评价体系。 2.4 实验四:六味地黄汤的药物反证大鼠分为模型组、六味地黄汤组和空白对照组。模型组和六味地黄汤组均
[Abstract]:Objective: the purpose of this subject is to explore the standard method of establishing animal model of kidney yin deficiency syndrome caused by thyroid hormone. At the same time, it provides a preliminary evaluation standard for the animal model of kidney yin deficiency, and provides a reference for the standardization of animal model of TCM syndrome. Second, method: 1. Theoretical study: the evaluation index selection of animal model of kidney yin deficiency syndrome 1.1 through consulting data summarized clinical kidney yin deficiency diagnosis criteria and objective index research results. 1.2 according to the clinical diagnostic criteria and evaluation indicators, the evaluation index of animal model of kidney yin deficiency syndrome was preliminarily developed. 2. Experimental study (divided into four steps) 2.1 experiment 1: primary screening of the rat model of kidney yin deficiency induced by thyroxine. In this experiment, SPF male Sprague-Dawley rats were selected. According to the most commonly used doses recorded in the literature, three doses of 1: 25 mg / kg bww 12.5 mg / kg bww 6.25 mg / kg BW were designed. Blood samples were collected continuously on the 14th day, 21 days and 28 days after modeling, respectively. The changes of serum cortisol (Cort), testosterone (T), estradiol (E _ 2), thyroxine T3 and T _ 4, camp and cGMP were observed. The dosage and time of administration were determined, compared with the blank control group, and compared with the control group, the dosage and time of administration were determined, and the changes of serum cortisol (Cort), testosterone (T), estradiol (E _ S _ 2), thyroxine T _ 3 and T _ 4, cyclic adenosine phosphate (camp) and cyclic guanosine phosphate (cGMP) were determined. 2.2 experiment 2: the method of preliminary screening thyroxine plus reserpine to make the model of kidney yin deficiency syndrome in rats. Three dose groups were designed according to the most commonly reported doses: thyroxine 37.5 mg / kg bww, reserpine 0.25 mg / kg bww, thyroxine 75 mg / kg bww, reserpine 0.5 mg / kg bww, thyroxine 150 mg / kg bww, reserpine 1.0mg/kgbw. Serum cortisol (Cort), testosterone (T), estradiol (E2), thyroxine T3 and T4, cyclic adenosine phosphate (camp), cyclic guanosine monophosphate (cGMP) and so on were observed in serum of animals of each dose group, and the changes of serum cortisol (Cort), testosterone (T), estradiol (E2), thyroxine T3 and T4, cyclic adenosine phosphate (camp) and cyclic guanosine monophosphate (cGMP) were observed. As compared with the blank control group, the dosage and time of administration were determined. 2.3 experiment 3: effects of thyroid hormone modeling on symptoms and other indexes in male SD rats According to the model making method selected from experiment 1, experiment 2, In the course of modeling, the general situation of animals was observed, and the endurance cold tolerance test, capture irritation test, isolation attack test, open field test and pregnancy rate of female rats were carried out, which were compared with the blank control group. Through the scientific observation of the symptoms, the deficiencies of the previous two experimental objective indicators were supplemented. And further improve the animal model of kidney yin deficiency syndrome symptom diagnosis and evaluation system. 2.4 experiment 4: Liuwei Dihuang decoction drug reverse syndrome Rats were divided into model group, Liuwei Dihuang decoction group and blank control group. Model group and Liuwei Dihuang decoction group
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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