日本血吸虫UV致弱尾蚴诱导小鼠免疫保护作用的研究

发布时间：2018-07-11 15:16

本文选题：C57BL/6 + 日本血吸虫　；参考：《南京医科大学》2006年硕士论文

【摘要】：目前，血吸虫病的防治工作强调以化疗为主，结合螺蛳控制、健康教育等手段来降低血吸虫病的危害。虽然，化疗在我国实施过程中起到了很好作用，但面临着再感染及抗药性等问题。鉴于疫苗在预防和消灭许多传染性疾病中发挥的重要作用，发展血吸虫病疫苗，从而使化疗“短效”作用与疫苗接种后产生的“长效”免疫预防作用相结合来控制血吸虫病，成为上个世纪80年代以来全世界科学家们的共识。减毒活疫苗如辐照致弱血吸虫尾蚴可在多种动物模型中诱导出高保护力，然而，，由于涉及诸如抗原材料来源有限、制备、保存和安全性等问题，导致了辐照致弱尾蚴应用的受限。研究者们希望通过揭示辐照致弱尾蚴诱导保护力的免疫机制，从而为新疫苗的设计提供理论依据。对曼氏血吸虫辐照致弱尾蚴诱导保护力和相关免疫应答机制的研究较多，其常用的小鼠品系有：C57BL／6、DBA、CBA等，其中，C57BL／6为一高应答品系。与曼氏血吸虫相比，日本血吸虫辐照致弱尾蚴在小动物(特别是小鼠)中诱导产生的保护力水平常不稳定，且目前缺乏公认的能对其产生稳定高应答的小鼠模型。目前，国内外对辐照致弱尾蚴的相关免疫学研究多集中在曼氏血吸虫，对日本血吸虫的研究则起步较晚，多在借鉴曼氏血吸虫病的相关研究经验的基础上开展。因此，构建对日本血吸虫辐照致弱尾蚴具有高保护力的小鼠模型，并动态观察日本血吸虫辐照致弱尾蚴诱导的免疫应答状况，对于探索辐照致弱尾蚴诱导保护力的免疫学机制至关
[Abstract]:At present, the prevention and control of schistosomiasis emphasizes chemotherapy mainly, combined with snail control, health education and other means to reduce the harm of schistosomiasis. Although chemotherapy has played a very good role in the implementation of China, it is facing the problems of reinfection and resistance to drug. In view of the importance of vaccine in the prevention and elimination of many infectious diseases The development of schistosomiasis vaccine, which makes the "short effect" effect of chemotherapy combined with the "long effect" immunization after vaccination to control schistosomiasis, has become the consensus of scientists all over the world since the 80s of the last century.
Attenuated live vaccines, such as irradiated weak schistosoma cercariae, can induce high protection in a variety of animal models. However, problems such as limited source of antigen, preparation, preservation and safety have led to the limitation of the application of irradiated cercariae. Researchers hope to pass through the immune system that reveals the protective ability of irradiated cercariae. It provides a theoretical basis for the design of the new vaccine.
There are many studies on the protective and related immune response mechanisms of cercariae induced by Schistosoma mansoni irradiation. The commonly used mice lines are C57BL / 6, DBA, CBA and so on. Among them, C57BL / 6 is a high response strain. Compared with Schistosoma mansoni, the protections induced by Schistosoma japonicum in small animals (especially mice) are induced by the irradiation of Schistosoma mansoni The level is often unstable, and there is no commonly accepted mouse model that can produce stable and high response to it.
At present, the related immunological studies of irradiated cercariae at home and abroad are mostly concentrated on Schistosoma mansoni, and the research on Schistosoma japonicum is relatively late, and it is based on the related research experience of mansoni schistosomiasis. Therefore, a mouse model with high protective ability to irradiate the weak cercariae caused by Schistosoma japonicum is constructed and the dynamic view is established. Observing the immune response induced by irradiated cercariae weakened by Schistosoma japonicum, it is very important to explore the immunological mechanism of radiation induced weak cercariae.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R383;R392

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