柯萨奇病毒A组16型临床分离株的生物学特性分析

发布时间：2018-07-12 12:28

本文选题：手足口病 + 柯萨奇病毒A组型　；参考：《中国生物制品学杂志》2014年05期

【摘要】：目的对引起手足口病(hand,foot and mouth disease,HFMD)的重要病原体柯萨奇病毒A组16型(coxsackievirus group A type16,CA16)临床分离株的生物学特性进行分析,为后续的疫苗研发奠定基础。方法从昆明地区HFMD临床疑似患者178份咽拭子及粪便标本中分离并鉴定CA16毒株,并对病毒的生长特性、蚀斑形态以及对乳鼠的致病性等生物学特性进行分析。结果共分离出7株CA16病毒:KM1、KM15、KM154、KM165、KM168、KM263、KM881,均属于B1亚型;病毒在Vero细胞上增殖较快,多数毒株4~6 d即可达到增殖高峰,但感染性滴度差别较大,最低为4.75 lgCCID50/ml,而最高可达7.78 lgCCID50/ml;各毒株在Vero细胞上培养相同时间形成的蚀斑形态不同,其中KM15、KM154、KM168蚀斑呈圆形,针尖样大小,边缘较清晰,KM1、KM881、KM263、KM165蚀斑较大,不规则,边缘较模糊;各毒株毒力均较强,经乳鼠颅内注射后,乳鼠均于3~6 d陆续开始发病,除KM168、KM154、KM15组乳鼠为不同程度的发病及死亡外,其他组乳鼠发病率及病毒致死性死亡率均达100%;各毒株对乳鼠的脑、心肌、肺、肌肉、脊髓和肝脏等组织均有不同程度的损伤,其中损伤较严重的组织为脑和肌肉。结论分离的CA16毒株对Vero细胞均具有良好的适应性,蚀斑清晰,毒力较强,且多数毒株感染性滴度较高,可用于CA16病毒致病机理的研究及疫苗的研发。
[Abstract]:Objective to analyze the biological characteristics of Coxsackie virus group A (Coxsackie virus) group 16 (coxsackievirus group A type16 CA16 clinical isolates causing hand foot and mouth disease (HFMD), so as to lay a foundation for further vaccine development. Methods CA16 strain was isolated and identified from 178 specimens of throat swabs and feces of suspected HFMD patients in Kunming area. The growth characteristics, plaque morphology and pathogenicity of CA16 virus were analyzed. Results A total of 7 strains of CA16 virus, KM154, KM165KM168KM263KM881, were isolated, and the virus proliferated rapidly on Vero cells, and most of the strains reached the peak of proliferation in 46 days, but the titer of infection was different. The lowest was 4.75 lgCCID 50 / ml, and the highest was 7.78 lgCCID50 / ml. The morphology of the plaque formed in Vero cells at the same time was different, in which KM15 KM154KM168 was round, needle size, edge was clear, KM881KM263KM165 was larger, irregular, and edge was blurry. The virulence of each strain was very strong. After intracerebral injection, the neonatal mice began to develop at 3d, except for the KM168KM154KM15 group, which had different degrees of morbidity and mortality. In other groups, the morbidity and mortality of the virus were 100%, and the brain, myocardium, lung, muscle, spinal cord and liver were damaged to different extent by each strain of virus, and the more serious ones were brain and muscle. Conclusion the isolated CA16 strains have good adaptability to Vero cells, clear plaque, strong virulence and high titer of infection, which can be used to study the pathogenic mechanism of CA16 virus and to develop vaccine.
【作者单位】：中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗工程技术研究中心云南省重大传染病疫苗研发重点实验室;
【基金】：云南省重点新产品开发计划项目(2012BC006)
【分类号】：R373.23

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