铜绿假单胞菌对致病真菌的抑制作用

发布时间：2018-07-13 09:19

【摘要】：目的观察临床分离的24株铜绿假单胞菌(PA)对致病性真菌(如白色念珠菌、热带念珠菌、光滑念珠菌、近平滑念珠菌、克柔念珠菌、葡萄牙念珠菌等)的抑制作用。方法收集临床上24株PA,通过革兰染色、氧化酶试验及API 20NE(法国梅里埃公司产品)进行鉴定;通过纸片法、十字交叉划线法及共培养检测PA对致病性真菌的抑制作用。十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对有抑制作用的PA和无抑制作用的PA分泌蛋白进行电泳。结果 24株PA中部分菌株对常见的致病性真菌有显著抑制作用,而部分PA菌株有一定抑制作用,另外一些完全没有抑制作用;共孵育试验显示PA可能是通过抑制真菌的菌丝生长而产生抑制作用。SDS-PAGE显示有抑制作用的PA和无抑制作用的PA分泌物蛋白间存在明显差异。结论PA对常见的致病真菌具有抑制作用,推测可能与抑制真菌的菌丝生长及分泌抑制真菌生长的各种蛋白类物质有关。
[Abstract]:Objective to observe the inhibitory effects of 24 strains of Pseudomonas aeruginosa (PA) on pathogenic fungi such as Candida albicans, Candida tropicalis, Candida smooth, Candida albicans, Candida krolimus, Candida Portugal, etc. Methods 24 strains of PAA were collected and identified by Gram staining, Oxidase test and API20NE. Sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to electrophoreze the inhibitory and non-inhibitory PA secreted proteins. Results some of the 24 strains of PA had a significant inhibitory effect on common pathogenic fungi, while some of the PA strains had a certain inhibitory effect, others had no inhibitory effect at all. The co-incubation test showed that PA was inhibited by inhibiting mycelium growth of fungi. SDS-PAGE showed that there was significant difference between PA with inhibitory effect and PA secretion without inhibition. Conclusion PA has an inhibitory effect on common pathogenic fungi, which may be related to the inhibition of mycelium growth and secretion of various proteins that inhibit the growth of fungi.
【作者单位】：华中科技大学同济医学院附属同济医院检验科;
【基金】：国家科技重大专项“十二五”资助课题(2012ZX10004207-004)
【分类号】：R378.991

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