不同年龄人群肌肉组织中7种线粒体酶的表达差异

发布时间：2018-07-13 12:30

【摘要】：目的:探讨年轻人与中老年人肌肉中线粒体电子传递链和胞质中一些关键酶的表达差异。方法:收集7例年轻人及33例中老年人颈部肌肉标本,透射电镜观察两组肌肉中线粒体形态学变化,免疫组织化学染色和蛋白质印迹检测NADH氧化还原酶辅酶1[NADH dehydrogenase(ubiquinone)1 alpha subcomplex 1,NDUFA1],琥珀酸脱氢酶复合体铁硫亚基(succinate dehydrogenase complex,subunit B,SDHB),细胞色素b(cytochrome b,Cytb),细胞色素C氧化酶(cytochrome C oxidase,COX),V型H+ATP酶(vacuolar-type H+-ATPase,V-ATPase H),苹果酸脱氢酶(cytosolic malate dehydrogenase,MDH)、短链羟烷基-辅酶A脱氢酶(hydroxyacyl-coenzyme A dehydrogenase,HADHSC)的表达情况。结果:与年轻人相比,中老年人肌肉中线粒体体积增大,空泡增多,嵴排列紊乱,出现类结晶状包涵体;NDUFA1、SDHB、Cytb、COX、V-ATPase H表达量明显降低(P均0.05),但MDH及HADHSC的表达差异无统计学意义;蛋白质印迹结果与免疫组织化学结果相一致。结论:肌肉组织中线粒体的形态和关键酶的表达随年龄的增加出现明显改变,可能在衰老过程中起到重要作用。
[Abstract]:Aim: to investigate the difference of expression of some key enzymes in mitochondria electron transport chain and cytoplasm between young and middle aged. Methods: the morphologic changes of mitochondria in the muscles of 7 young and 33 middle aged patients were observed by transmission electron microscope (TEM). Immunohistochemical staining and Western blot analysis of nadh dehydrogenase (ubiquinone) 1 alpha subcomplex 1 (NDUFA1), succinic dehydrogenase complex (succinate dehydrogenase complex subunit (succinate dehydrogenase, cytochrome b (cytochrome BX, cytochrome C oxidase, vacuolar-type H ATPase H), (cytosolic malate dehydrogenase (MDH) and hydroxyacyl-coenzyme A dehydrogenase (HADHSC). Results: compared with the young, the volume of mitochondria, vacuoles increased, the ridge arranged disordered, and the expression of COXV-ATPase H in the muscle of the middle and old people decreased obviously (P 0.05), but the expression of MDH and HADHSC was not significantly different. The results of Western blot were consistent with those of immunohistochemistry. Conclusion: the morphology of mitochondria and the expression of key enzymes in muscle tissue change obviously with the increase of age, which may play an important role in the process of aging.
【作者单位】：江苏大学附属人民医院耳鼻喉科;江苏大学附属人民医院普外科;江苏大学附属人民医院中心实验室;
【基金】：江苏省六大人才高峰项目(2011-WSN021D) 江苏省医学科研招标立项课题(H201353) 镇江市科技支撑社会发展项目(SH2012042)
【分类号】：R339.38

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