维生素C-预处理减弱缺氧对豚鼠左心室肌细胞钠电流的损伤影响

发布时间：2018-07-18 10:41

【摘要】： 目的:研究缺氧及缺氧前使用Vitamin C(1mM)预处理对心室肌细胞瞬时性钠电流及持续性钠电流的影响,以期探讨缺氧时Vitamin C的保心作用机理。方法:使用膜片钳全-细胞和单-通道技术记录缺氧及缺氧前使用Vitamin C预处理后心肌细胞快钠电流(I_(NaT))和持续性钠电流(I_(NaP))的变化,研究在缺氧条件下Vitamin C对心室肌细胞钠电流的保护性作用。结果:全-细胞记录数据显示,在给灌流心肌细胞持续维持缺氧状态15分钟过程中,缺氧实验组中I_(NaT)(瞬时性钠电流)的平均电流密度从对照值的40.2142±1.7735减小到27.1663±1.8441pA/pF而I_(NaP)(持续性钠电流)的平均电流密度从对照值0.3987±0.0474增大至1.1854±01994pA/pF(n=9, P0.05 vs.对照组)。然而,当在缺氧前向冲浴池内加入Vitamin C(1.0mM)预处理后,I_(NaT) and I_(NaP)的电流参数仅发生轻度地改变:I_(NaT)的平均电流密度从对照值的41.6038±2.9762减小到34.6341±1.9651pA/pF而I_(NaP)的平均电流密度从对照值0.3843±0.0636增大至0.6734±0.1057pA/pF(n=9, P0.05 vs.缺氧组)。另一方面,单通道(细胞-贴附式)记录显示:在缺氧组和Vitamin C-预处理组中,I_(NaP)的平均开放概率和开放时间均出现增大,但是Vitamin C-预处理组中I_(NaP)的电流参数增大程度比缺氧组要明显的小。结论:我们的实验结果显示,维生素C能有效地削弱缺氧对豚鼠心室肌细胞钠电流( I_(NaT)和I_(NaP))的损伤作用。
[Abstract]:Aim: to study the effects of Vitamin C (1m) pretreatment on transient sodium currents and persistent sodium currents in ventricular myocytes before and after hypoxia. Methods: the changes of fast sodium current (I _ (NaT) and persistent sodium current (I _ (NaP) in cardiac myocytes were recorded by patch clamp whole-cell and single-channel techniques before and after anoxia and anoxic treatment with Vitamin C. To study the protective effect of Vitamin C on sodium current in ventricular myocytes under hypoxia. Results: Full-cell records showed that during 15 minutes of continuous hypoxia in perfused cardiomyocytes, The average current density of I _ (NaT) (transient sodium current) decreased from 40.2142 卤1.7735 to 27.1663 卤1.8441 Pa / PF, and the average current density of I _ (Na) (persistent sodium current) increased from 0.3987 卤0.0474 to 1.1854 卤0.1994 Pa / PF (n9,P0.05 vs). Control group). However, after pretreatment with Vitamin C (1.0mm) in anoxic shower pool, the current parameters of INaT and INaP decreased slightly from 41.6038 卤2.9762 to 34.6341 卤1.9651pAppF, and the average current density of INaT increased from 0.3843 卤0.0636 to 0.6734 卤0.1057pAP-pF (NNM, P0.05 vs). Hypoxia group) On the other hand, single channel (cell-attached) records showed that the mean open probability and opening time of INaP increased in hypoxia and Vitamin C- preconditioning groups. However, the I _ (NAP) current parameters in the Vitamin C-preconditioning group were significantly smaller than those in the hypoxia group. Conclusion: our results show that vitamin C can effectively attenuate the effects of hypoxia on sodium currents (I _ (NaT) and I _ (NaP) in guinea pig ventricular myocytes.
【学位授予单位】：武汉科技大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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