黑色素瘤抗原-4 DNA疫苗的构建及体内抑瘤效应的研究

发布时间：2018-07-20 09:35

【摘要】： 本研究将人黑色素瘤抗原-4(MAGE-4)基因克隆入真核表达载体pcDNA3.1+，构建真核表达载体pcDNA3.1+/MAGE-4，并对其表达进行鉴定。将pcDNA3.1+/MAGE-4转染小鼠结肠癌细胞CT26，建立表达人MAGE-4的鼠CT26细胞株。将质粒pcDNA3.1+/MAGE-4扩增后，作为核酸疫苗对小鼠进行肌肉注射，，观察其对小鼠细胞免疫和体液免疫的影响及对肿瘤的治疗和预防作用。结果表明，经质粒pcDNA3.1+/MAGE-4免疫治疗的小鼠其脾淋巴细胞CTL杀伤活性及脾淋巴细胞上清液中IL-2和IFN-γ明显强于或高于对照组，两者相比有显著性差异。在接种肿瘤细胞的小鼠中，经pcDNA3.1+/MAGE-4免疫治疗后能产生明显的预防和治疗肿瘤的作用。本研究提示MAGE-4作为肿瘤靶抗原可诱导产生明显的细胞免疫应答和体液免疫应答，对肿瘤的生成和发展有治疗和预防的作用。
[Abstract]:In this study, the human melanoma antigen 4 (MAGE-4) gene was cloned into eukaryotic expression vector pcDNA3.1, and the eukaryotic expression vector pcDNA3.1 / MAGE-4 was constructed and identified. A mouse CT26 cell line expressing human MAGE-4 was established by transfection of pcDNA3.1 / MAGE-4 into mouse colon cancer cell line CT26. The plasmid pcDNA3.1% MAGE-4 was amplified and injected intramuscularly into mice as nucleic acid vaccine. The effects of pcDNA3.1% MAGE-4 on cellular and humoral immunity and the therapeutic and preventive effects on tumor were observed. The results showed that the CTL killing activity of spleen lymphocytes and IL-2 and IFN- 纬 in the supernatant of splenic lymphocytes of mice immunized with pcDNA3.1 / MAGE-4 were significantly higher than or higher than those of the control group. In mice inoculated with tumor cells, immunotherapy with pcDNA3.1 / MAGE-4 could significantly prevent and treat tumor. This study suggests that MAGE-4, as a tumor target antigen, can induce obvious cellular and humoral immune responses, and has a therapeutic and preventive effect on tumor formation and development.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R739.5;R392

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