腺嘌呤制备阳虚型慢性肾功能衰竭大鼠模型综合指标及其机理研究

发布时间：2018-07-20 12:47

【摘要】：研究目的：本课题通过动物实验，对相关检验指标研究，开发适用于防治阳虚型慢性肾功能衰竭中医药药理学研究实用、稳定的动物模型，，探讨阳虚型慢性肾功能衰竭SD大鼠模型的客观化指标、病理机制及其与人类CRF病变的异同。材料与方法：采用健康SD大鼠24只，正常饲养7天后，随机分为腺嘌呤灌胃模型组(12只)、空白对照组(12只)。腺嘌呤灌胃模型组：按200mg／kg·d剂量将腺嘌呤制成混悬液2ml，隔日1次，定时经灌胃给药，累计总剂量为3g／只，全过程30d；空白对照组：正常饲料喂养，全过程30d。全部动物均自由摄取饲料及饮水。两组动物在第31天采用常规麻醉后处死，经腹主动脉取血10ml做相关实验室检查，取双肾按光镜、电镜病理学要求取样送检。选择肾脏病理学、血液生化(肾功、肝功、电解质)、一氧化氮(NO)、内皮素(ET)、环磷酸鸟苷(cGMP)、环磷酸腺苷(cAMP)、IgG、IgM、血栓素(TXB2)、前列腺素(6-K—PGFla)、肿瘤坏死因子(TNF-a)、白介素(IL-1β)及中分子物质(MMS)等客观指标进行研究，统计分析各项指标在实验中的变化及其意义，综合阐述腺嘌呤制备阳虚型慢性肾功能衰竭动物模型的发病机理，及其与人类慢性肾功能衰竭病理改变的异同。结果： 1 临床表现：模型组SD大鼠在用药12-15d开始出现多尿、精神萎靡、眼睑周围浮肿、体重增长缓慢等一系列阳虚型肾功能衰竭临床表现，空白组外观及活动正常，体重上升。 2 模型组SD大鼠肾功能检测与空白对照组比较差异性显著(P＜0.01)，但尿酸含量空白组大于模型组； 3 模型组SD大鼠电解质检测与空白对照组比较，钾、磷差异性显著(P＜0.01)，钠、钙、氯化物均无明显差异(P＞0.05)；
[Abstract]:Objective: to develop a practical and stable animal model of TCM pharmacology for the prevention and treatment of chronic renal failure due to Yang deficiency through animal experiments. Objective: to explore the objective indexes, pathological mechanism and the differences and similarities between SD rat model of chronic renal failure with yang deficiency and human CRF. Materials and methods: 24 healthy SD rats were randomly divided into adenine perfusion model group (n = 12) and blank control group (n = 12) after 7 days of normal feeding. In the model group, adenine was made into suspension at the dose of 200mg/kg d, once every other day. The cumulative total dose of adenine was 3g/ only for 30 days, while the blank control group was fed with normal diet for 30 days. All animals are free to eat feed and drinking water. The animals of the two groups were killed after routine anesthesia on the 31st day. Blood 10ml was taken from the abdominal aorta for laboratory examination. The two kidneys were taken for sampling according to light microscope and electron microscope pathology. Choose renal pathology, blood biochemistry (renal function, liver function), Electrolyte, nitric oxide (no), endothelin (et), cyclic guanosine monophosphate (cGMP), cyclic adenosine monophosphate (camp) IgGG, thromboxane (TXB2), prostaglandin (6-K-PGFla), tumor necrosis factor (TNF-a), interleukin-1 尾 (IL-1 尾) and mesomolecular substance (MMS) were studied. The changes of various indexes in the experiment and their significance were analyzed. The pathogenesis of the animal model of chronic renal failure caused by adenine deficiency and the similarities and differences between the pathological changes of chronic renal failure and that of human chronic renal failure were expounded. Results: 1 Clinical manifestations: SD rats in the model group began to develop polyuria, mental depression and edema around the eyelid at 12-15 days after treatment. A series of clinical manifestations of renal failure of Yang deficiency type, such as slow weight gain, normal appearance and activity in blank group, 2 the renal function of SD rats in the model group was significantly different from that in the blank control group (P < 0.01), but the uric acid content in the blank group was higher than that in the model group. (3) compared with the control group, the electrolyte of SD rats in the model group showed significant difference in potassium and phosphorus (P < 0.01), but there was no significant difference in sodium, calcium and chloride (P > 0.05).
【学位授予单位】：福建中医学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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