超分子免疫磁珠定量PCR技术的建立及大肠癌转移相关标志物的探讨
发布时间：2018-07-28 07:41
【摘要】：大肠癌是一种常见恶性肿瘤，发病率呈逐年上升的趋势，目前在我国恶性肿瘤中列第五位、在欧美发达国家列第三位。尽管近些年临床对大肠癌的诊治水平有明显提高，但大肠癌的病死率仍居高不下，其主要原因是大肠癌较难被早期发现，大部分患者到中晚期才被发现，错过了手术的最佳时机，复发和转移仍是患者死亡的主要原因。因此，阐明大肠癌发生和转移机制、发现新的大肠癌特异性标志物、研究新的标志物检测技术以及对标志物的作用机制的研究成为目前该领域研究的热点课题。 大肠癌是在环境和遗传因素共同作用下，经历多基因，多步骤、多阶段复杂的生物学过程演变而来。大肠癌发生发展的经典模式是：结直肠上皮细胞增生—腺瘤—非典型增生—癌—转移癌。在大肠癌发病过程的不同阶段，，肿瘤细胞由于基因组改变产生一些肿瘤相关和／或肿瘤特异的小分子蛋白质／肽，分泌某些细胞因子或相关抗体，这些物质可释放到血液中，形成大肠癌血清肿瘤标志物。目前已证实有许多癌基因和抑癌基因如环氧化酶2基因、P53基因等在大肠癌发病过程中发生显著的变化，这些基因产物分泌到血液中，便可成为某些特异大肠癌血清标志物。但由于血清是一种十分复杂和多样化的液态基质，尽管大肠癌发生后可导致血清蛋白在结构和数量上发生某些特征性的变化，但由于目前我们对血清蛋白的了解有限，只有很少一部分血清蛋白如癌胚抗原(CEA)、乳糖蛋白系列(CA125，CA199)用于常规的临床诊断，而其它大肠癌相关基因产物如环氧化酶
[Abstract]:Colorectal cancer is a common malignant tumor, the incidence of which is increasing year by year. At present, colorectal cancer ranks fifth among malignant tumors in China and third in developed countries in Europe and America. Although the clinical diagnosis and treatment of colorectal cancer has improved significantly in recent years, the fatality rate of colorectal cancer is still high. The main reason is that colorectal cancer is difficult to be detected early, and most patients are not discovered until the middle and late stages. Missed the optimal time for surgery, recurrence and metastasis is still the main cause of death. Therefore, to clarify the mechanism of colorectal cancer occurrence and metastasis, to find new specific markers of colorectal cancer, to study new marker detection techniques and the mechanism of action of markers has become a hot topic in this field. Colorectal cancer is a complex biological process with multiple genes, steps and stages under the combined action of environment and genetic factors. The classic pattern of colorectal carcinogenesis and progression is colorectal epithelial cell proliferation-adenoma-atypical hyperplasia-carcinoma-metastasis. At different stages of the development of colorectal cancer, tumor cells produce small tumor-related and / or tumor-specific proteins / peptides that secrete cytokines or antibodies that can be released into the blood as a result of genomic changes. The serum tumor markers of colorectal cancer were formed. It has been proved that many oncogenes and tumor suppressor genes such as cyclooxygenase-2 gene and p53 gene have changed significantly in the pathogenesis of colorectal cancer. These gene products secreted into the blood can become some specific colorectal cancer serum markers. However, because the serum is a very complex and diverse liquid matrix, although the occurrence of colorectal cancer can lead to some characteristic changes in the structure and quantity of serum protein, due to the current limited understanding of serum protein, Only a few serum proteins, such as carcinoembryonic antigen (CEA), lactoglycoprotein series (CA125, CA199), are used for routine clinical diagnosis, while other colorectal cancer related gene products such as cyclooxygenase
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R735.34;R392
本文编号：2149387
[Abstract]:Colorectal cancer is a common malignant tumor, the incidence of which is increasing year by year. At present, colorectal cancer ranks fifth among malignant tumors in China and third in developed countries in Europe and America. Although the clinical diagnosis and treatment of colorectal cancer has improved significantly in recent years, the fatality rate of colorectal cancer is still high. The main reason is that colorectal cancer is difficult to be detected early, and most patients are not discovered until the middle and late stages. Missed the optimal time for surgery, recurrence and metastasis is still the main cause of death. Therefore, to clarify the mechanism of colorectal cancer occurrence and metastasis, to find new specific markers of colorectal cancer, to study new marker detection techniques and the mechanism of action of markers has become a hot topic in this field. Colorectal cancer is a complex biological process with multiple genes, steps and stages under the combined action of environment and genetic factors. The classic pattern of colorectal carcinogenesis and progression is colorectal epithelial cell proliferation-adenoma-atypical hyperplasia-carcinoma-metastasis. At different stages of the development of colorectal cancer, tumor cells produce small tumor-related and / or tumor-specific proteins / peptides that secrete cytokines or antibodies that can be released into the blood as a result of genomic changes. The serum tumor markers of colorectal cancer were formed. It has been proved that many oncogenes and tumor suppressor genes such as cyclooxygenase-2 gene and p53 gene have changed significantly in the pathogenesis of colorectal cancer. These gene products secreted into the blood can become some specific colorectal cancer serum markers. However, because the serum is a very complex and diverse liquid matrix, although the occurrence of colorectal cancer can lead to some characteristic changes in the structure and quantity of serum protein, due to the current limited understanding of serum protein, Only a few serum proteins, such as carcinoembryonic antigen (CEA), lactoglycoprotein series (CA125, CA199), are used for routine clinical diagnosis, while other colorectal cancer related gene products such as cyclooxygenase
【学位授予单位】：第一军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R735.34;R392
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相关期刊论文 前3条
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2 王征,阮幼冰,官阳;肝细胞癌患者血清蛋白质组成分的双向凝胶电泳-飞行时间质谱分析[J];中华病理学杂志;2003年04期
3 王立顺,尹艳慧,高素君,殷剑宁,赵晓航,朱迅;以双向凝胶电泳为基础的食管癌组织蛋白质组学分析新策略[J];中国免疫学杂志;2003年04期
本文编号：2149387
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