B类Ⅰ型清道夫受体在小型猪及HepG2、THP-1细胞中表达的变化

发布时间：2018-07-29 13:12

【摘要】：B类Ⅰ型清道夫受体(scavenger receptor class B type I,SR-BI)是第一个在分子水平上证实的位于细胞膜表面的高密度脂蛋白(high density lipoprotein,HDL)受体。SR-BI有多种配体,它可以介导胆固醇和其他脂质在HDL和细胞之间进行转运。逆向胆固醇转运(reverse cholesterol transport,RCT)是HDL介导的一种从外周组织向肝脏转运多余胆固醇的过程。RCT在体内有多个步骤,其中第一步就是肝外组织细胞中的未酯化胆固醇流出到HDL,而最后一步则是HDL中的胆固醇酯经选择性吸收而转运到肝脏的过程。作为HDL的唯一受体,SR-BI在RCT中起着十分重要的作用。本研究拟在动脉粥样硬化小型猪模型上观察肝脏和血管SR-BI表达的变化,然后采用ox-LDL、LDL及HDL分别处理肝HepG2细胞和肝外THP-1巨噬细胞,观察细胞水平上SR-BI的表达及细胞内胆固醇转运的变化,为进一步阐明SR-BI在动脉粥样硬化发生发展中的作用提供实验依据。第一部分:动脉粥样硬化小型猪SR-BI表达的变化目的:探讨动脉粥样硬化小型猪肝脏和主动脉组织中SR-BI表达的变化。方法:采用饲喂高脂高胆固醇饲料的贵州小香猪为研究对象,喂养12个月后处死动物,采用油红O和HE染色检测肝脏蓄脂情况及动脉粥样斑块病变,逆转录-聚合酶链反应(RT-PCR)、Western印迹和免疫组织化学法分别检测肝脏和主动脉组织中SR-BImRNA和蛋白质的表达。结果:油红O及HE染色可见动脉粥样硬化小型猪肝脏有大量的脂肪空泡,动脉有明显的脂质条纹及斑块。采用RT-PCR、Western印迹与免疫组织化学分别检测组织SR-BImRNA和蛋白质的表达,结果显示,与对照组小型猪比较,动脉粥样硬化小型
[Abstract]:Type B scavenger receptor, (scavenger receptor class B type Igno SR-BI, is the first type of type B scavenger receptor. SR-BI has multiple ligands on the surface of the cell membrane, and is the first confirmed receptor at the molecular level. It mediates the transport of cholesterol and other lipids between HDL and cells. Reverse cholesterol transport (reverse cholesterol transporter (RCTs) is a HDL mediated transport of excess cholesterol from peripheral tissues to the liver. RCTs have multiple steps in vivo. The first step is the flow of unesterified cholesterol from extrahepatic tissue cells to HDLs, and the last step is the selective absorption and transport of cholesterol esters from HDL to the liver. SR-BI, the sole receptor of HDL, plays an important role in RCT. The purpose of this study was to observe the changes of SR-BI expression in liver and blood vessels of atherosclerotic miniature pigs, and then to treat liver HepG2 cells and extrahepatic THP-1 macrophages with ox-LDLL-LDL and HDL, respectively. The expression of SR-BI and the changes of intracellular cholesterol transport were observed in order to further elucidate the role of SR-BI in the pathogenesis and development of atherosclerosis. Part one: changes of SR-BI expression in atherosclerotic miniature pigs objective: to investigate the changes of SR-BI expression in liver and aorta of atherosclerotic miniature pigs. Methods: Guizhou Xiao Xiang pig fed with high fat and high cholesterol diet was used as the research object. After 12 months of feeding, the animals were killed. The liver lipid accumulation and atherosclerotic plaque were detected by oil red O and HE staining. Reverse transcription-polymerase chain reaction (RT-PCR) Western blot and immunohistochemistry were used to detect the expression of SR-BImRNA and protein in liver and aorta respectively. Results: oil red O and HE staining showed that there were a lot of fat vacuoles in the liver of atherosclerotic miniature pigs and obvious lipid stripes and plaques in the arteries. RT-PCR Western blot and immunohistochemistry were used to detect the expression of SR-BImRNA and protein respectively. The results showed that compared with the control group, atherosclerosis was small.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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