TOCP诱发母鸡OPIDN发生过程中神经电生理、抗氧化系统及凋亡相关蛋白变化

发布时间：2018-07-31 06:04

【摘要】：目的研究三邻甲苯基磷酸酯(Tri-ortho-cresyl phosphate，TOCP)诱发母鸡迟发性神经毒性(organophosphorus easter-induced delayed neurotoxicity，OPIDN)发生过程中(1)坐骨神经动作电位(compound action potention parameters，CAP)特性的时间-效应关系及其与症状分级间的关系；(2)中枢和周围神经系统组织及血清中氧化抗氧化系统时间-效应关系及其与症状分级间的关系；(3)神经组织中凋亡相关蛋白cyt-c表达的时间-效应关系，寻找敏感指标，为探讨TOCP引起OPIDN的发病机制及早期诊断提供依据。方法 1．建立动物模型：成年罗曼母鸡，随机分为d0，5，10，15和21，5个时间点。TOCP溶解于玉米油中，给药组母鸡一次po 750mg.kg~(-1) TOCP(0.65ml／kg)，，对照组(d0)给予同体积的玉米油。 2．迟发性神经毒性临床症状观察：观察各组母鸡的一般状况变化，定期测量母鸡体重，每天观察母鸡的OPIDN临床表现至实验结束，按8级评价神经毒性。 3．痛觉阈值及坐骨神经动作电位参数测定：分别测定d0，5，10，15和21试验动物的痛觉阈值，坐骨神经传导速度，CAP潜伏期、波幅、最大刺激强度和阈强度。 4．神经组织和血清中脂质过氧化及抗氧化能力指标的测定：分别测定d0，5，10，15和21大脑、脊髓、坐骨神经和血清中丙二醛(malondialdehyde，MDA)和谷胱甘肽(glutathione，GSH)含量、谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)活性、谷胱甘肽还原酶(glutathione reductase，GR)活性、超氧化物歧化酶(superoxide dismutase，SOD)活性、抗活性氧(anti-reactive oxygen
[Abstract]:Objective to study the time-effect relationship of the characteristics of sciatic nerve action potential (compound action potention parametersCAP) in organophosphorus easter-induced delayed neurotoxicity induced by Tri-ortho-cresyl phosphate TOCP and its relationship with symptom grading in hens. (2) the time-effect relationship of oxidative antioxidant system in central and peripheral nervous system and serum and its relationship with symptom grading, (3) the time-effect relationship of cyt-c expression of apoptosis-related protein in nerve tissue, and the search for sensitive indexes. To investigate the pathogenesis and early diagnosis of OPIDN caused by TOCP. Methods 1.Establishment of animal model: adult Roman hens were randomly divided into five time points. TOCP was dissolved in corn oil. The hens in the administration group received 750 mg 路kg ~ (-1) TOCP (0.65ml/kg once, while the control group (day 0) was given the same volume of corn oil. 2. Observation of the clinical symptoms of delayed neurotoxicity: the observation of each group Changes in the general state of the hens in the group, The weight of hens was measured regularly, and the clinical manifestations of OPIDN were observed every day until the end of the experiment. The threshold of pain and the parameters of sciatic nerve action potential were measured. The conduction velocity of sciatic nerve in the latent period and amplitude of CAP, Maximum stimulation intensity and threshold intensity. 4. Determination of lipid peroxidation and antioxidant capacity in nerve tissue and serum. The contents of malondialdehyde (MDA) and glutathione (GSH), the activity of glutathione peroxidase (GSH-Px) and the activity of glutathione reductase (glutathione reductase gr) in sciatic nerve and serum were measured. Superoxide Dismutase (superoxide) activity and Anti-reactive oxygen species (anti-reactive oxygen) activity
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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