表皮葡萄球菌mscL基因突变株的构建及其生物学功能
发布时间：2018-07-31 15:43
【摘要】：目的初步探讨表皮葡萄球菌mscL基因的生物学功能。方法构建含有壮观霉素抗性基因(spc)的同源重组质粒pMAD-ΔmscL,电转入表皮葡萄球菌1457,在42℃条件下振荡培养,利用蓝白斑和抗生素抗性筛选表皮葡萄球菌mscL基因敲除突变株(SE1457-ΔmscL)。通过检测低渗胁迫前后突变株和野生株D600值及CFU的变化观察mscL基因对渗透压的调节作用。采用微量板半定量方法检测不同条件下mscL敲除突变对细菌生物膜形成的影响。结果成功构建同源重组质粒pMAD-ΔmscL,经PCR扩增和测序验证获得了表皮葡萄球菌mscL基因敲除突变株,并通过RT-PCR在转录水平上得到进一步验证。处于对数生长期的mscL突变株在低渗胁迫下的生存能力显著降低,但其生物膜形成能力与野生株相比无显著差异。结论表皮葡萄球菌mscL基因可在对数生长期参与渗透压的调节,但对生物膜的形成无影响。
[Abstract]:Objective to investigate the biological function of mscL gene in Staphylococcus epidermidis. Methods the homologous recombinant plasmid pMAD- 螖 mscL containing spectinomycin resistance gene (spc) was constructed and transferred into Staphylococcus epidermidis 1457. The mutant SE1457- 螖 mscL). Was screened by blue and white spot and antibiotic resistance at 42 鈩，
本文编号：2156046
[Abstract]:Objective to investigate the biological function of mscL gene in Staphylococcus epidermidis. Methods the homologous recombinant plasmid pMAD- 螖 mscL containing spectinomycin resistance gene (spc) was constructed and transferred into Staphylococcus epidermidis 1457. The mutant SE1457- 螖 mscL). Was screened by blue and white spot and antibiotic resistance at 42 鈩，
本文编号：2156046
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