C型利钠尿肽对小鼠Cajal间质细胞起博电流的影响

发布时间：2018-08-02 10:41

【摘要】：C型利尿钠肽对小鼠Cajal间质细胞起搏电流的影响 1893年，西班牙神经解剖学家cajial用甲基蓝及嗜银染色法在胃肠道神经系统(enteric nervous system，ENS)中观察到一类特殊的间质细胞，命名为Cajal间质细胞(interstitial cells of Cajal，ICC)。ICC是ENS中的一种非神经但与神经有关的特殊间质细胞，胃肠道平滑肌的运动形式在很大程度上取决于ICC的活动。已有众多研究表明，许多胃肠道平滑肌的静息电位不稳定，表现为缓慢的起伏波动，，即周期性去极化和复极化，称为慢波(slow wave，SW)，也称基本电节律(Basic Electric Rhythm，BER)。BER决定着平滑肌收缩节律，调控着胃肠道运动发生的时间、地点、频率、和方向。近年来的单细胞记录和分子水平的研究证明，胃肠道无论是纵行肌还是环行肌均不具有发动SW的能力。慢波由存在于纵行肌和环行肌之间的ICC产生，这些细胞具有成纤维细胞和平滑肌细胞的特性，并与纵环两层形成缝隙连接，可将慢波传到平滑肌。近年的许多研究表明，ICC是肠道慢波的起搏者，在神经肌肉信号传递中起调节作用，而且与某些胃肠动力性疾病的发病有关。利尿钠肽(natriuretic peptides)家族是一类具有利尿、排钠，舒张血管，降低血压以及调节水盐和电解质平衡等作用的多肽。利尿钠肽家族的受体有3种，分别是A型、B型和C型利尿钠肽受体(natriuretic peptides receptors，NPR)。利尿钠肽通过与其受体结合而发挥作用。CNP作为利尿钠肽家族中的一员，是由Sudoh等人于1990年首先在猪的大脑中发现的，是一种具有22个氨基酸残基的肽类：CNP分布广泛，存在于中枢神经系统、心血管系统、泌尿生殖系统、消化系统以及皮肤和粘膜等，几乎遍布于整个机体。CNP作为一种局部调节因子通过自分泌和旁分泌的方式发挥作用，除了可以利尿、排钠，舒张血管和内脏平滑肌，降低血压以及调节水盐、电解质平衡以外，还可以抑制血管平滑肌的增殖调节肾素血管紧张素的活性和调节肠液和胆汁等消化液的分泌。此外，还参与高血压、慢性心衰、慢性肾衰、动脉粥样硬化和肺纤维化等一系列病理过程的调节。CNP的许多作用是通过调节细胞膜上的离子通道，影响细胞内信号转导来实现的。我室以往的研究表明：在大鼠胃的各个部位都有利尿钠肽受体(natriuretic
[Abstract]:Effects of C-type diuretic natriuretic peptide on pacing current of mouse Cajal interstitial cells in 1893, Cajial, a Spanish neuroanatomist, observed a special type of interstitial cells in the gastrointestinal (enteric nervous system by methylene blue and argyrophilic staining. Cajal interstitial cells (interstitial cells of Cajal ICC). ICC is a kind of special interstitial cells in ENS, which is non-nerve but related to nerve. The motility of gastrointestinal smooth muscle depends on the activity of ICC to a great extent. Many studies have shown that the resting potential of gastrointestinal smooth muscle is unstable, which is characterized by slow fluctuation, that is, periodic depolarization and repolarization, known as slow wave SW, also known as basic electrical rhythm (Basic Electric Rhythm-BER, which determines the contraction rhythm of smooth muscle. It regulates the time, place, frequency, and direction of gastrointestinal movements. In recent years, single cell recording and molecular studies have proved that gastrointestinal tract, whether longitudinal or circular, does not have the ability to initiate SW. The slow waves are produced by ICC which exists between longitudinal and circular muscles. These cells possess the characteristics of fibroblasts and smooth muscle cells and form a gap junction with the longitudinal ring to transmit the slow waves to the smooth muscle. In recent years, many studies have shown that ICC is a slow wave pacemaker, which plays a regulatory role in neuromuscular signal transduction, and is related to the pathogenesis of some gastrointestinal motility diseases. Diuretic natriuretic peptide (natriuretic peptides) family is a family of polypeptides with diuretic, natriuretic, vasodilating, lowering blood pressure, and regulating the balance of water, salt and electrolyte. There are three kinds of diuretic natriuretic peptide receptor, type A, type B, and type C, (natriuretic peptides receptor. Diuretic natriuretic peptide acts by binding to its receptor. CNP, as a member of the diuretic natriuretic peptide family, was first discovered in the pig brain by Sudoh et al in 1990. Present in the central nervous system, cardiovascular system, genitourinary system, digestive system, skin and mucous membrane, almost all over the body. CNP acts as a local regulatory factor through autocrine and paracrine. In addition to diuretic, sodium excretion, vasodilator and visceral smooth muscle, lowering blood pressure and regulating water, salt, electrolyte balance, It can also inhibit the proliferation of vascular smooth muscle, regulate the activity of renin angiotensin and regulate the secretion of digestive fluid such as intestinal fluid and bile. In addition, CNP is involved in the regulation of a series of pathological processes such as hypertension, chronic heart failure, chronic renal failure, atherosclerosis and pulmonary fibrosis. Previous studies in my laboratory have shown that diuretic natriuretic peptide receptors (natriuretic) are present in all parts of the rat stomach.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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