中国广东汉族与新疆维吾尔族24个Y-STR基因座的多态性分析

发布时间：2018-08-02 14:21

【摘要】：目的应用GFS 24Y试剂盒,对中国广东汉族和新疆维吾尔族共584个无关男性样本进行24个Y-STR基因座分型,统计其基因座、单倍型的遗传多态性,并分析其群体差异。方法采用磁珠法提取样本DNA后用GFS24Y试剂盒扩增,3130XL遗传分析仪进行基因分型,根据分型结果进行统计分析。结果获得了中国广东汉族与新疆维吾尔族共584个样本24个Y-STR基因座的分型结果及各等位基因频率分布资料。在广东汉族群体中,各基因座的基因多样性(GD值)为0.560 6~0.963 9,由24个基因座组成的单倍型共有299个,单倍型多样性(HD值)为0.999 93;在新疆维吾尔族中,GD值为0.443 9~0.957 9,共观察到282个单倍型,HD值为0.999 85。两个民族未观察到共有单倍型。通过两个群体间的比较,共有22个Y-STR基因座等位基因频率分布在两个民族之间存在差异(P0.05),各基因座在两个民族的遗传分化系数(Fst值)为0.000 767 9(DYS481)~0.247 946(DYS520)。结论 24个Y-STR基因座在中国广东汉族与新疆维吾尔族人群中均有较高的多态性,并显示出明显的民族特征,在法医亲权鉴定和个体识别、群体遗传学等领域均有重要的应用价值。
[Abstract]:Objective using GFS 24Y kit, 24 Y-STR loci were genotyped from 584 unrelated male samples from Guangdong Han and Xinjiang Uygur nationalities, and the genetic polymorphisms of their loci and haplotypes were analyzed and their population differences were analyzed. Methods DNA was extracted by magnetic bead method and then amplified by GFS24Y kit for genotyping by 3130XL genetic analyzer. Results the results of 24 Y-STR loci of 584 samples of Han nationality and Xinjiang Uygur nationality in Guangdong, China, and the distribution of allelic frequencies were obtained. In Guangdong Han population, the genetic diversity (GD value) of each locus was 0.560 60.9639, and there were 0.560 haplotypes composed of 24 loci. The diversity of haplotypes (HD value) was 0.999 93, and the GD value in Xinjiang Uygur nationality was 0.443 990.957 9. A total of 282 haplotypes were observed with HD value of 0.999 85. No haplotypes were observed between the two peoples. The allele frequencies of 22 Y-STR loci were different between the two populations (P0.05). The genetic differentiation coefficient (Fst) of each locus was 0.000 7679 (DYS481) 0.247,946 (DYS520). Conclusion the 24 Y-STR loci were highly polymorphic in Han and Xinjiang Uygur populations in Guangdong and Xinjiang, and showed obvious ethnic characteristics. They were identified by forensic paternity test and individual identification. Population genetics and other fields have important application value.
【作者单位】：中山大学中山医学院法医学系;广州市公安局白云分局;广州市刑事科学技术研究所;
【基金】：国家自然科学基金(81273347) 公安部应用创新计划项目(2011YYCXGDST076) 广东省科技计划项目(2010A060801001)
【分类号】：R394

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