肌成纤维细胞在大鼠排卵机制中的作用
发布时间：2018-08-03 19:03
【摘要】：目的： 1．确认在大鼠卵巢卵泡膜中有肌成纤维细胞存在 2．探讨肌成纤维细胞与卵巢排卵的关系 方法： 1．采用免疫组织化学方法检测α-SMA、desmin、vimentin、ET-AR和ET-BR在动情期组(n=20)与非动情期组(n=10)大鼠卵巢各级卵泡(窦前卵泡、小窦状卵泡和大窦状卵泡)卵泡膜细胞中的表达情况，并进行图像分析。 2．采用透射电镜技术观察动情期大鼠窦状卵泡卵泡膜细胞的超微结构。 结果： 1．免疫组织化学结果： 1．1．α-SMA表达于动情期和非动情期各级卵泡的卵泡膜细胞中，其表达强度在组间同级卵泡间比较有显著性差异(P＜0.05)，组内各级卵泡间比较也有显著性差异(P＜0.05)；在动情期组其表达强度与卵泡直径大小成正相关(r=0.631)，而非动情期组两者却几乎无相关性(r=-0.087)。 1．2．Desmin在动情期组各级卵泡的卵泡膜细胞中表达，其表达强度在该组各级卵泡间比较有显著性差异(P＜0.05)：在非动情期组各级卵泡的卵泡膜细胞中无表达。 1．3．Vimentin表达于动情期和非动情期各级卵泡的卵泡膜细胞中，其表达强度在组间同级卵泡间比较有显著性差异(P＜0.05)。动情期组各级卵泡间比较有显著性差异(P＜0.05)而且vimentin表达强度与卵泡直径大小呈正相关(r=0.536)；非动情期组窦前卵泡和小窦状卵泡间比较无明显差异(P＞0.05)，其他级别卵泡间比较有显著性差异(P＜0.05)，而且vimentin表达强度与卵泡
[Abstract]:Objective: 1. To confirm the presence of myofibroblasts in the follicular membrane of rat ovary 2. To investigate the relationship between myofibroblasts and ovulation. 1.Immunohistochemical method was used to detect the ovarian follicles (preantral follicles) in the estrus group (nong20) and the non-estrous group (nm10). The expression of follicular membrane cells in antral follicles and large antral follicles, The ultrastructure of antral follicular membrane cells in estrus was observed by transmission electron microscopy. Results: 1. Immunohistochemical results: 1.1. 伪 -SMA was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was a significant difference in the expression intensity between the same grade follicles (P < 0.05) and among the follicles of different levels in the group (P < 0.05). There was a positive correlation between the expression intensity and follicle diameter in estrus group (r 0. 631), but almost no correlation between the expression intensity and follicle diameter in non estrus group (r 0. 087). 1.2.Desmin was active in oestrus. In the oestrous stage group, the follicular membrane cells of all levels of follicles were expressed, There was significant difference in the expression intensity among the follicles (P < 0.05), and there was no expression in the theca cells of the follicles in the non-estrous period group. 1.3.Vimentin was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was significant difference in expression intensity between the same grade follicles (P < 0.05). In estrus group, there was significant difference among follicles (P < 0. 05), and there was a positive correlation between vimentin expression and follicular diameter (r = 0. 536). There was no significant difference between preantral follicle and antral follicle in non-estrous stage group (P > 0. 05), but there was significant difference between other grade follicles (P < 0. 05), and the expression intensity of vimentin was higher than that of follicle group (P < 0. 05).
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321
本文编号：2162710
[Abstract]:Objective: 1. To confirm the presence of myofibroblasts in the follicular membrane of rat ovary 2. To investigate the relationship between myofibroblasts and ovulation. 1.Immunohistochemical method was used to detect the ovarian follicles (preantral follicles) in the estrus group (nong20) and the non-estrous group (nm10). The expression of follicular membrane cells in antral follicles and large antral follicles, The ultrastructure of antral follicular membrane cells in estrus was observed by transmission electron microscopy. Results: 1. Immunohistochemical results: 1.1. 伪 -SMA was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was a significant difference in the expression intensity between the same grade follicles (P < 0.05) and among the follicles of different levels in the group (P < 0.05). There was a positive correlation between the expression intensity and follicle diameter in estrus group (r 0. 631), but almost no correlation between the expression intensity and follicle diameter in non estrus group (r 0. 087). 1.2.Desmin was active in oestrus. In the oestrous stage group, the follicular membrane cells of all levels of follicles were expressed, There was significant difference in the expression intensity among the follicles (P < 0.05), and there was no expression in the theca cells of the follicles in the non-estrous period group. 1.3.Vimentin was expressed in follicular membrane cells of all levels of follicles in estrus and non-estrous stage. There was significant difference in expression intensity between the same grade follicles (P < 0.05). In estrus group, there was significant difference among follicles (P < 0. 05), and there was a positive correlation between vimentin expression and follicular diameter (r = 0. 536). There was no significant difference between preantral follicle and antral follicle in non-estrous stage group (P > 0. 05), but there was significant difference between other grade follicles (P < 0. 05), and the expression intensity of vimentin was higher than that of follicle group (P < 0. 05).
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321
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本文编号：2162710
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