贺斯在体外对健康人免疫系统细胞因子的影响
发布时间：2018-08-03 17:48
【摘要】：目的 探讨不同浓度的羟乙基淀粉类血浆代用品(贺斯,6%HAES 200/0.5)对正常人体免疫系统白介素-2,10,12(IL-2,10,12)及干扰素-γ(IFN-γ)的影响,以检验其是否抑制正常人体的免疫系统,为在围术期替代输血提供试验依据,以降低输血造成的恶性肿瘤的复发率及术后感染率和病死率。 方法 正常志愿者12名,严格无菌条件下抽取静脉血各20毫升,并以肝素抗凝,在超净台内分离出单个核细胞,计数后将细胞平均分成5组:1640培养液阴性对照组、10%血浆对照组、10%贺斯试验组、20%贺斯试验组、40%贺斯试验组,然后再将各组分成阴性对照组、加脂多糖(LPS)组、加植物血凝素(PHA)组,使单个核细胞在不同的培养液中培养48小时后,离心提取上清液,以酶联免疫吸附法(ELISA法)检测各上清液中细胞因子白介素-2,10,12(IL-2,IL-10,IL-12)和干扰素-γ(IFN-γ)的浓度。 结果 三个浓度的贺斯试验组与1640培养液及血浆对照组在三种环境(阴性对照、加LPS、加PHA)中比较,虽然四种细胞因子的浓度值稍有参差,但统计学显示无显著性差异(p0.05);在IL-2和IFN-γ,阴性对照组与加LPS组无显著性差异(p0.05),而两者均与加PHA组有显著性差异(p0.05);在IL-10,IL-12,阴性对照组、加LPS组及加PHA组之间均示有显著性差异(p0.05)。 结论 三个浓度的贺斯下培养单个核细胞48小时后与同条件下的血浆组及阴性培养液组的细胞因子浓度无显著差异,提示三个浓度的贺斯在体外环境中不影响免疫细胞的生存、克隆及细胞因子的产生,即:贺斯在体外试验
[Abstract]:Objective to investigate the effects of different concentrations of hydroxyethyl starch plasma substitutes (HES 200 / 0.5) on the levels of interleukin-2siloxacin 1012 (IL-2m10N12) and interferon- 纬 (IFN- 纬) in normal human immune system in order to determine whether they inhibit the immune system of normal human beings. In order to reduce the recurrence rate, postoperative infection rate and mortality of malignant tumor caused by blood transfusion, we can provide experimental basis for replacing blood transfusion in perioperative period. Methods Mononuclear cells were isolated from 12 normal volunteers under strict aseptic conditions by extracting 20 ml of venous blood and using heparin as anticoagulant. After counting, the cells were divided into 5 groups, 10% plasma control group, 10% Horse test group and 40% Horse test group, then each group was divided into negative control group, lipopolysaccharide (LPS) group and phytohemagglutinin (PHA) group. After the mononuclear cells were cultured in different culture medium for 48 hours, the supernatants were extracted by centrifugation. The concentrations of cytokines interleukin-210-10 (IL-12) and interferon- 纬 (IFN- 纬) in the supernatants were detected by enzyme-linked immunosorbent assay (ELISA). Results the concentrations of four cytokines were slightly different between the three concentrations of Horse test group and 1640 medium and plasma control group in three environments (negative control, plus LPS, plus PHA), although the concentrations of four cytokines were slightly different. In IL-2 and IFN- 纬, there was no significant difference between the negative control group and the LPS group (p0.05), but there was a significant difference between the two groups (p0.05), and there was a significant difference between the IL-10 IL-12 group, the negative control group, the LPS group and the PHA group (p0.05). Conclusion there was no significant difference in cytokine concentrations between the three concentrations of Hos cultured mononuclear cells in vitro and plasma and negative medium groups after 48 hours, suggesting that the three concentrations of Hos did not affect the survival of immune cells in vitro. Cloning and production of cytokines, I. e., Hoss in vitro
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392.1
本文编号：2162499
[Abstract]:Objective to investigate the effects of different concentrations of hydroxyethyl starch plasma substitutes (HES 200 / 0.5) on the levels of interleukin-2siloxacin 1012 (IL-2m10N12) and interferon- 纬 (IFN- 纬) in normal human immune system in order to determine whether they inhibit the immune system of normal human beings. In order to reduce the recurrence rate, postoperative infection rate and mortality of malignant tumor caused by blood transfusion, we can provide experimental basis for replacing blood transfusion in perioperative period. Methods Mononuclear cells were isolated from 12 normal volunteers under strict aseptic conditions by extracting 20 ml of venous blood and using heparin as anticoagulant. After counting, the cells were divided into 5 groups, 10% plasma control group, 10% Horse test group and 40% Horse test group, then each group was divided into negative control group, lipopolysaccharide (LPS) group and phytohemagglutinin (PHA) group. After the mononuclear cells were cultured in different culture medium for 48 hours, the supernatants were extracted by centrifugation. The concentrations of cytokines interleukin-210-10 (IL-12) and interferon- 纬 (IFN- 纬) in the supernatants were detected by enzyme-linked immunosorbent assay (ELISA). Results the concentrations of four cytokines were slightly different between the three concentrations of Horse test group and 1640 medium and plasma control group in three environments (negative control, plus LPS, plus PHA), although the concentrations of four cytokines were slightly different. In IL-2 and IFN- 纬, there was no significant difference between the negative control group and the LPS group (p0.05), but there was a significant difference between the two groups (p0.05), and there was a significant difference between the IL-10 IL-12 group, the negative control group, the LPS group and the PHA group (p0.05). Conclusion there was no significant difference in cytokine concentrations between the three concentrations of Hos cultured mononuclear cells in vitro and plasma and negative medium groups after 48 hours, suggesting that the three concentrations of Hos did not affect the survival of immune cells in vitro. Cloning and production of cytokines, I. e., Hoss in vitro
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392.1
【参考文献】
相关期刊论文 前10条
1 董晓军;白细胞介素-10检测及其临床意义[J];标记免疫分析与临床;2001年04期
2 王珊娟;血浆代用品的临床应用进展[J];国外医学.麻醉学与复苏分册;1995年02期
3 徐雪;人工血浆代用品对凝血功能的影响[J];国外医学.麻醉学与复苏分册;2001年02期
4 徐从高,管增伟;IL-12抗肿瘤活性的研究进展[J];国外医学(肿瘤学分册);1996年06期
5 袁世荧,姚尚龙,夏磊铭,卫富强;羟乙基淀粉溶液对手术病人血浆细胞因子的影响[J];临床麻醉学杂志;2002年08期
6 邓硕曾,刘进;建议中华麻醉学会制定输血指南[J];临床麻醉学杂志;1999年03期
7 李文硕;血浆代用品的临床应用[J];天津护理;2002年05期
8 王军,任久林,魏继东;白细胞介素与心血管疾病研究进展[J];医学综述;2002年12期
9 马骐,张涛;最新一代血浆代用品——中分子羟乙基淀粉(贺斯)[J];The Chinese-German Journal of Clinical Oncology;1999年04期
10 张进玉，，杨世勇;细胞因子与临床[J];中级医刊;1996年01期
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