SGA大鼠生后早期骨骼发育相关因素研究

发布时间：2018-08-06 08:42

【摘要】：第一部分能量控制法大鼠IUGR模型的建立【目的】探讨能量控制方法建立大鼠IUGR模型的效果,为研究SGA大鼠早期生长发育提供研究对象。【方法】选取70天日龄、体重为220～265克的成熟未孕Wistar大鼠,以常规饲养方法喂养,试验时按雌雄2:1比例合笼交配,次晨取阴道分泌物涂片查精子,查见精子确定为受孕0天。将受孕鼠23只随机分为限食组和对照组,另选取未孕鼠12只作为未孕限食组。限食组从受孕开始控制进食量,食物量为对照组前一天进食量的一半,直到分娩,分娩后即恢复到自由进食。孕前和孕期每周测量体重。鼠仔生后由母鼠喂养,出生时和生后每3天测量体重和身长。【结果】限食组和对照组的孕鼠体重均呈现正性增长,限食组的体重增长明显低于对照组,而未孕限食组体重却呈负增长。限食组和对照组所产鼠仔数量没有差异。以对照组鼠仔出生体重作为参照,限食组IUGR鼠的发生率为51.01%。限食组鼠仔体重在12天以前、身长在15天以前与对照组差异明显,之后就没有差异。限食组鼠仔生后1周以内体重增长低于对照组、身长增长与对照组无差异,之后体重身长增长都快于对照组。【结论】中度食物限制明显影响孕期的体重增长且怀孕状态下的营养代谢模式与非孕期明显不同,可能与孕期体内激素水平的变化有关。在较好条件下饲养的大鼠受孕时身体储备较好,早期的中度食物控制对受孕没有大的影响,一旦受孕,中度食物控制不会增加胚胎和胎鼠的死亡。全程中度食物限制明显影响
[Abstract]:Part one: the establishment of IUGR model in rats with energy control method [objective] to investigate the effect of energy control method on establishing rat IUGR model. In order to study the early growth and development of SGA rats, 70 days old Wistar rats weighing 220 ~ 265g were selected and fed with conventional feeding methods. In the experiment, the female and male were mated in a cage at 2:1, and the vaginal secretion smear was taken in the morning to check the sperm, and the sperm was determined to be pregnant for 0 days. Twenty-three pregnant rats were randomly divided into restricted group and control group, and 12 non-pregnant rats were selected as control group. The food intake of the restricted group was half of that of the control group the day before, and then returned to free eating after delivery. Weight is measured before and during pregnancy. The body weight and body length were measured at birth and every 3 days after birth. [results] the body weight of pregnant mice in diet restricted group and control group showed positive increase. The weight gain of the restricted diet group was significantly lower than that of the control group, but the weight of the non pregnant diet restricted group was negative. There was no difference in litter size between the diet restricted group and the control group. The birth weight of rats in control group was taken as reference. The incidence of IUGR in diet restricted group was 51. 01%. The body weight of the rats in the diet restricted group was 12 days ago, and the body length was 15 days before the control group, but there was no difference between the diet control group and the control group. The gain of body weight in the diet restricted group was lower than that in the control group within 1 week after birth, and there was no difference in the growth of body length between the control group and the control group. [conclusion] moderate food restriction significantly affected the weight gain during pregnancy and nutritional metabolic patterns during pregnancy. [conclusion] moderate food restriction significantly affects the nutritional metabolic patterns during pregnancy. [conclusion] moderate food restriction significantly affects the weight gain during pregnancy and the nutritional metabolic pattern during pregnancy. Unlike non-pregnant women, It may be associated with changes in hormone levels during pregnancy. The rats fed under better conditions had better body reserves during pregnancy, but early moderate food control had no significant effect on pregnancy. Once pregnant, moderate food control did not increase the mortality of embryo and fetus. Significant effects of moderate food restriction during the whole course
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R-332

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