移动电话电磁辐射对小鼠生殖功能的影响
发布时间：2018-08-06 14:02
【摘要】：【第一部分】 移动电话电磁辐射对雄性小鼠生殖功能的影响 目的:探讨移动电话电磁辐射对雄性小鼠生殖系统功能的影响。 方法:用移动电话的模拟辐射源对雄性昆明小鼠进行全身辐射,辐射频率为935MHz,时间为2h/d,连续35d,按平均功率密度分为3个组,即0μW/cm~2组(对照组)、570μW/cm~2辐射组和1400μW/cm~2辐射组。辐射后取双侧睾丸和附睾称重,采用全自动生化分析仪检测睾丸组织乳酸脱氢酶同工酶(lactic dehydrogenase-x isozyme,LDH-X)活性,常规分析附睾的精子计数、活动率和畸形率,并观察睾丸组织结构及电镜超微结构。 结果:(P) LDH-X活性:1400μW/cm~2辐射组的LDH-X活性为(338.143±73.348)U/g,明显低于570μW/cm~2辐射组[(401.780±50.919)U/g]和对照组[(408.449±71.325)U/gl,差异均有显著性(P0.05);570μW/cm~2辐射组和对照组比较,差异无显著性(P0.05)。(2) 精子的常规分析:1400μW/cm~2辐射组的精子活动率为(40.818±4.729)%,与对照组[(47.667±6.964)%]比较,差异有显著性(P0.05);570μW/cm~2辐射组的精子活动率[(44.800±6.102)%]与对照组比较,差异无显著性(P0.05)。精子计数和精子畸形率在各组间的差异均无显著性(P0.05)。(3) 组织结构和超微结构:①各辐射组的睾丸组织结构未见异常。②1400μW/cm~2辐射组出现精子尾部线粒体的超微结构改变,表现为形态大小不一,分布不均;部分出现肿胀,电子密度降低;板状嵴减少,灶性空化。570μW/cm~2辐射组的超微结构未见明显改变。 结论:在本实验条件下,1400μW/cm~2的移动电话电磁辐射可引起LDH-X活性降低、精子活动率下降及精子尾部线粒体的超微结构改变,提示一定强度的移动电话电磁辐射可能影响雄性生殖功能,其机制可能是通过作用于线粒体、阻碍生精细胞(尤其是精子)的能量代谢而产生生殖毒性。
[Abstract]:Effect of electromagnetic radiation of mobile phone on reproductive function of male mice objective: to study the effect of electromagnetic radiation of mobile phone on male mice The influence of the function of colonization system. Methods: the simulated emitter of mobile phone was used to radiate the male Kunming mice. The radiation frequency was 935 MHz and the time was 2 h / d for 35 days. According to the average power density, the mice were divided into three groups: 0 渭 W/cm~2 group (control group) and 1400 渭 W/cm~2 radiation group. After irradiation, the testis and epididymis were weighed, and the activity of lactic dehydrogenase-x isozyme LDH-X in testis was measured by automatic biochemical analyzer. The sperm count, motility rate and abnormal rate of epididymis were analyzed routinely. The structure of testis and ultrastructure of electron microscope were observed. Results: the activity of (P) LDH-X in the 1 400 渭 W/cm~2 radiation group was (338.143 卤73.348) U / g, which was significantly lower than that in the 570 渭 W/cm~2 radiation group [(401.780 卤50.919) U / g] and the control group [(408.449 卤71.325) U / g]. The difference was significant (P0.05) between the 570 渭 W/cm~2 radiation group and the control group. There was no significant difference (P0.05). (2) in the sperm motility rate of the 1 400 渭 W/cm~2 radiation group (40.818 卤4.729), which was significantly different from that of the control group [(47.667 卤6.964)%] (P0.05). The sperm motility rate of the 570渭 W/cm~2 radiation group [(44.800 卤6.102)%] was not significantly different from that of the control group (P0.05). There was no significant difference in sperm count and sperm deformity rate among the three groups (P0.05). (3). There were no significant changes in testicular tissue structure in the radiation group of 0.21400 渭 W/cm~2 and the ultrastructure of spermatozoa tail mitochondria in the radiation group (P0.05). (3). The results showed that the morphology was different, the distribution was uneven, some swelling appeared, the electron density was decreased, the plate ridge was decreased, and the ultrastructure of focal cavitation. 570 渭 W/cm~2 radiation group had no obvious change. Conclusion: under the condition of this experiment, the electromagnetism radiation of 1 400 渭 W/cm~2 of mobile phone can reduce the activity of LDH-X, decrease the rate of sperm motility and change the ultrastructure of mitochondria in the tail of spermatozoa. The results suggest that electromagnetic radiation of mobile phone may affect male reproductive function, and its mechanism may be reproductive toxicity by acting on mitochondria and blocking the energy metabolism of spermatogenic cells (especially sperm).
【学位授予单位】：武汉大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363
[Abstract]:Effect of electromagnetic radiation of mobile phone on reproductive function of male mice objective: to study the effect of electromagnetic radiation of mobile phone on male mice The influence of the function of colonization system. Methods: the simulated emitter of mobile phone was used to radiate the male Kunming mice. The radiation frequency was 935 MHz and the time was 2 h / d for 35 days. According to the average power density, the mice were divided into three groups: 0 渭 W/cm~2 group (control group) and 1400 渭 W/cm~2 radiation group. After irradiation, the testis and epididymis were weighed, and the activity of lactic dehydrogenase-x isozyme LDH-X in testis was measured by automatic biochemical analyzer. The sperm count, motility rate and abnormal rate of epididymis were analyzed routinely. The structure of testis and ultrastructure of electron microscope were observed. Results: the activity of (P) LDH-X in the 1 400 渭 W/cm~2 radiation group was (338.143 卤73.348) U / g, which was significantly lower than that in the 570 渭 W/cm~2 radiation group [(401.780 卤50.919) U / g] and the control group [(408.449 卤71.325) U / g]. The difference was significant (P0.05) between the 570 渭 W/cm~2 radiation group and the control group. There was no significant difference (P0.05). (2) in the sperm motility rate of the 1 400 渭 W/cm~2 radiation group (40.818 卤4.729), which was significantly different from that of the control group [(47.667 卤6.964)%] (P0.05). The sperm motility rate of the 570渭 W/cm~2 radiation group [(44.800 卤6.102)%] was not significantly different from that of the control group (P0.05). There was no significant difference in sperm count and sperm deformity rate among the three groups (P0.05). (3). There were no significant changes in testicular tissue structure in the radiation group of 0.21400 渭 W/cm~2 and the ultrastructure of spermatozoa tail mitochondria in the radiation group (P0.05). (3). The results showed that the morphology was different, the distribution was uneven, some swelling appeared, the electron density was decreased, the plate ridge was decreased, and the ultrastructure of focal cavitation. 570 渭 W/cm~2 radiation group had no obvious change. Conclusion: under the condition of this experiment, the electromagnetism radiation of 1 400 渭 W/cm~2 of mobile phone can reduce the activity of LDH-X, decrease the rate of sperm motility and change the ultrastructure of mitochondria in the tail of spermatozoa. The results suggest that electromagnetic radiation of mobile phone may affect male reproductive function, and its mechanism may be reproductive toxicity by acting on mitochondria and blocking the energy metabolism of spermatogenic cells (especially sperm).
【学位授予单位】：武汉大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363
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