人类基因遗传标志物（STR，HLA）与男性长寿关联性的研究
发布时间：2018-08-11 09:04
【摘要】： 目的一般认为长寿的机理是多种因素、综合作用的结果,不是单一因素所决定的。人类的长寿现象更多的是一个体与外界的生存环境相适应的结果。在与长寿有关的因素中,遗传因素无疑是最重要或最主要的因素之一。人类基因组计划的研究表明,人类基因中99.9%的遗传密码DNA都是一样的,只有大约0.1%的基因不同,具有个体差异。因此,与人类长寿有关的遗传因素很可能与这0.1%个体差异相关。目前,人类最大的遗传多态性是人类白细胞抗原系统(Human Leukocyte Antigen, HLA)和微卫星DNA(short tandem repeat,STR)。因此本文选定HLA-I类基因和15个STR系统的等位基因,旨在研究与人类长寿相关的遗传因素。 方法选取大连地区,年龄在90岁以上的男性老人18人,作为观察组。对照组随机选取大连地区无偿献血志愿者男性50名,身体健康,体检标准符合“全国采供血管理规范”。家族无遗传病史,年龄在18-55周岁。 样本采集,采静脉血2ml,EDTA抗凝。HLA-A、B基因分型方法,采用SSO方法。首先,提取DNA,进行PCR扩增。然后,按试剂盒说明书进行变性与中和后再与磁珠杂交,用SAPE染色,洗涤后,读板,计算机自动按顺序读取数据并分析出A、B、DR的特异性。 STR检测采用Identifiler PlusTM (ABI公司,美国)试剂盒,扩增位点为D8S1179、D21S11、D7S820、CSF1PO、D3S1358、THO1、D13S317、D16S539、D2S1338、D19S433、VWA、TPOX、D18S51、D5S818、FGA和Amelogenin性别位点;采用310型DNA
[Abstract]:Objectives It is generally believed that the mechanism of longevity is determined by many factors and not by a single factor. Human longevity is more likely to be the result of adaptation to the external environment. Among the factors related to longevity, genetic factors are undoubtedly one of the most important or most important. Studies have shown that 99.9% of the genetic code DNA in human genes is the same, only about 0.1% of the genes are different and have individual differences. And microsatellite DNA (short tandem repeat, STR). Therefore, HLA-I and 15 STR alleles were selected to study the genetic factors related to human longevity.
Methods Eighteen male elderly people over 90 years old in Dalian area were selected as the observation group. Fifty male volunteers in Dalian area were randomly selected as the control group.
Sample collection, venous blood 2 ml, EDTA anticoagulant, HLA-A, B genotyping method, using SSO method. First, extract DNA, PCR amplification. Then, according to the kit instructions for denaturation and neutralization and then hybridization with magnetic beads, stained with SAPE, washed, read the board, computer automatically read the data in order to analyze the specificity of A, B, DR.
STR was detected by Identifiler PlusTM (ABI Company, USA) kit. The amplified sites were D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA and Amelogenen. Gender loci 310 were used.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R394
本文编号：2176530
[Abstract]:Objectives It is generally believed that the mechanism of longevity is determined by many factors and not by a single factor. Human longevity is more likely to be the result of adaptation to the external environment. Among the factors related to longevity, genetic factors are undoubtedly one of the most important or most important. Studies have shown that 99.9% of the genetic code DNA in human genes is the same, only about 0.1% of the genes are different and have individual differences. And microsatellite DNA (short tandem repeat, STR). Therefore, HLA-I and 15 STR alleles were selected to study the genetic factors related to human longevity.
Methods Eighteen male elderly people over 90 years old in Dalian area were selected as the observation group. Fifty male volunteers in Dalian area were randomly selected as the control group.
Sample collection, venous blood 2 ml, EDTA anticoagulant, HLA-A, B genotyping method, using SSO method. First, extract DNA, PCR amplification. Then, according to the kit instructions for denaturation and neutralization and then hybridization with magnetic beads, stained with SAPE, washed, read the board, computer automatically read the data in order to analyze the specificity of A, B, DR.
STR was detected by Identifiler PlusTM (ABI Company, USA) kit. The amplified sites were D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA and Amelogenen. Gender loci 310 were used.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R394
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