青春双歧杆菌对环磷酰胺免疫抑制小鼠的免疫增强作用

发布时间：2018-08-12 13:07

【摘要】： 目的:用环磷酰胺腹腔注射造成免疫抑制小鼠动物模型,研究青春双歧杆菌在体内对免疫抑制小鼠的免疫增强作用。方法:先用环磷酰胺给正常小鼠腹腔注射连续4天,出现免疫抑制状态后,再用青春双歧杆菌菌液3种不同浓度干预治疗免疫抑制小鼠,同时设黄芪治疗组,正常生理盐水对照组和单纯免疫抑制组,每组又分为7天治疗和14天治疗,分别测各组小鼠血中IL-2含量,胸腺、脾脏、肝脏指数,小鼠体重增长曲线,粪便青春双歧杆菌(Bifido adolescentis,BFA)菌落计数,血白细胞数、胸腺、肝、脾HE梁色切片,采用SPSS12.0统计软件包进行数据处理分析,统计学方法采用双因素重复测量资料的方法分析单因素随机化设计的方差分析,卡方检验,多组均数比较的非参数秩和检验。结果:随着治疗天数的增加,BFA粪便菌落数逐渐减少,双歧杆菌高剂量组、生理盐水组、单纯免疫抑制组比较有统计学意义,提示BFA随摄入增多,肠道定植也增多;根据肝、脾、胸腺指数的比较结果,说明BFA对免疫器官有一定增重作用;血IL-2,白细胞含量比较,BFA高剂量组最高,提示双歧杆菌对免疫抑制小鼠免疫细胞分泌IL-2,以及白细胞生成有一定促进作用,体重曲线也表现出高剂量组双歧杆菌对体重促进作用最明显,组织HE染色形态观察也提示双歧杆菌对胸腺、肝、脾免疫淋巴组织有恢复作用。结论:双歧杆菌对环磷酰胺免疫抑制小鼠有一定的免疫增强作用。
[Abstract]:Aim: to establish an immunosuppressive mouse model by intraperitoneal injection of cyclophosphamide and to study the immunoenhancement effect of Bifidobacterium pubertidis on immunosuppressive mice in vivo. Methods: normal mice were injected intraperitoneally with cyclophosphamide for 4 days, and then treated with three different concentrations of Bifidobacterium pubescens solution. The mice were treated with astragalus membranaceus (Astragalus membranaceus) at the same time. Normal saline control group and immunosuppressive group, each group was divided into 7 days treatment and 14 days treatment. Blood IL-2 content, thymus, spleen, liver index, weight growth curve of mice in each group were measured, respectively. Fecal Bifido adolescentis bacillus colony count, white blood cell count, thymus, liver, spleen HE color sections were analyzed by SPSS12.0 statistical software package. Statistical methods were used to analyze the ANOVA, chi-square test, and nonparametric rank sum test of multivariate mean comparison in single factor randomization design. Results: with the increase of treatment days, the fecal colony number of BFA gradually decreased. There were significant differences among the high dose bifidobacterium group, normal saline group and immunosuppressive group, suggesting that BFA increased with the intake, intestinal colonization also increased, according to liver, spleen, The comparison of thymus index indicated that BFA had a certain weight gain effect on immune organs, and the contents of IL-2 and WBC were the highest in high dose group. It was suggested that Bifidobacterium could promote the secretion of IL-2 and leukocyte formation in immunosuppressive mice, and the body weight curve showed that Bifidobacterium in high dose group had the most obvious effect on body weight. The morphological observation of HE staining also showed that Bifidobacterium could recover the immune lymphoid tissue of thymus, liver and spleen. Conclusion: Bifidobacterium has a certain immunoenhancement effect on cyclophosphamide immunosuppressive mice.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R371

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