TNF-α IL-1β和IL-6影响细菌生长的体外实验研究
发布时间：2018-08-15 13:01
【摘要】：前言 无论是以个体或组织成分存在的细胞,均对其环境的信号做出反应,并由此建立细胞间的信息传递。在维持组织稳态和防御感染或非感染性损伤的宿主反应中,细胞以自分泌或旁分泌方式借助于低分子量多肽-细胞因子调节其生长和增殖。感染是病原体与宿主间相互作用的结果。研究表明,感染引起的全身性炎症反应综合征(SIRS)和器官损伤中,病原体或其产物可能仅是触发或始动因素,而机体对致炎因素的应答可能更为重要。人们首先注意到,感染性疾病时宿主应答的一个重要方式是通过释放前炎性细胞因子,产生有利或不利于感染控制的效应;而近年来也开始注意到病原体可能利用或逃避宿主产生的细胞因子,并做了初步研究。在TNF-α、IL-1β和IL-6与感染性疾病的研究中发现,这些细胞因子适量分泌有利于控制感染,过度产生则对机体不利;它们的浓度与疾病的严重程度和预后相关;细胞因子持续升高者更易发生院内感染。关于病原体可能利用或逃避宿主免疫防御的研究中,有些学者已发现某些细胞因子能促进某些细菌生长,但相关机理不清楚,很多问题有待研究。本实验选择了与炎症关系最为密切的细胞因子-TNF-α、IL-1β和IL-6,研究其对院内感染中最常见的病原菌-绿脓杆菌、金黄色葡萄球菌和不动杆菌体外生长的影响,探讨细菌和宿主细胞因子之间的相互作用,为迁延性炎症中细菌增殖的机制提供新的依据,也为评价旨在防止宿主防御反应失调时细胞因子紊乱为切入点的治疗手段提供进一步的实验室证据。 实验材料和方法 一、实验材料
[Abstract]:Both individual and tissue components respond to signals from their environment and establish intercellular information transmission. In maintaining tissue homeostasis and host response against infection or non-infectious injury, cells autocrine or paracrine regulate their growth and proliferation by means of low molecular weight polypeptide cytokines. Infection is the result of the interaction between pathogen and host. Studies have shown that pathogens or their products may be only triggers or initiators in (SIRS) and organ damage caused by infection, but the response of organism to inflammatory factors may be more important. It is first noted that an important way of host response in infectious diseases is to produce beneficial or unfavorable effects on infection control by releasing preinflammatory cytokines. In recent years, we have also noticed that pathogens may use or escape the cytokines produced by the host, and have made a preliminary study. In the study of TNF- 伪 IL-1 尾, IL-6 and infectious diseases, it was found that the proper secretion of these cytokines was beneficial to control infection, but excessive production was harmful to the body, their concentration was related to the severity and prognosis of the disease. Increased cytokines are more likely to cause nosocomial infection. Some researchers have found that some cytokines can promote the growth of some bacteria, but the related mechanism is not clear, and many problems need to be studied. In this study, cytokines TNF- 伪, IL-1 尾 and IL-6, which are most closely related to inflammation, were selected to study the effects of TNF- 伪 IL-1 尾 and IL-6 on the growth of Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter Acinetobacter, the most common pathogens of nosocomial infection, in vitro. The study of the interaction between bacteria and host cytokines provides a new basis for the mechanism of bacterial proliferation in persistent inflammation. It also provides further laboratory evidence for the evaluation of therapeutic approaches aimed at preventing cytokine disorders in host defense response disorders. Experimental materials and methods
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
本文编号：2184280
[Abstract]:Both individual and tissue components respond to signals from their environment and establish intercellular information transmission. In maintaining tissue homeostasis and host response against infection or non-infectious injury, cells autocrine or paracrine regulate their growth and proliferation by means of low molecular weight polypeptide cytokines. Infection is the result of the interaction between pathogen and host. Studies have shown that pathogens or their products may be only triggers or initiators in (SIRS) and organ damage caused by infection, but the response of organism to inflammatory factors may be more important. It is first noted that an important way of host response in infectious diseases is to produce beneficial or unfavorable effects on infection control by releasing preinflammatory cytokines. In recent years, we have also noticed that pathogens may use or escape the cytokines produced by the host, and have made a preliminary study. In the study of TNF- 伪 IL-1 尾, IL-6 and infectious diseases, it was found that the proper secretion of these cytokines was beneficial to control infection, but excessive production was harmful to the body, their concentration was related to the severity and prognosis of the disease. Increased cytokines are more likely to cause nosocomial infection. Some researchers have found that some cytokines can promote the growth of some bacteria, but the related mechanism is not clear, and many problems need to be studied. In this study, cytokines TNF- 伪, IL-1 尾 and IL-6, which are most closely related to inflammation, were selected to study the effects of TNF- 伪 IL-1 尾 and IL-6 on the growth of Pseudomonas aeruginosa, Staphylococcus aureus and Acinetobacter Acinetobacter, the most common pathogens of nosocomial infection, in vitro. The study of the interaction between bacteria and host cytokines provides a new basis for the mechanism of bacterial proliferation in persistent inflammation. It also provides further laboratory evidence for the evaluation of therapeutic approaches aimed at preventing cytokine disorders in host defense response disorders. Experimental materials and methods
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392
【引证文献】
相关硕士学位论文 前1条
1 杨磊;奶牛乳腺炎乳汁三种细胞因子检测及TNF-α对乳腺细胞凋亡和增殖的影响[D];内蒙古农业大学;2010年
，本文编号：2184280
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