缺氧预处理对骨髓来源内皮祖细胞生物学功能的影响

发布时间：2018-08-19 19:52

【摘要】：目的研究缺氧条件对骨髓来源内皮祖细胞(BM-EPCs)生物学功能的影响。方法经密度梯度离心法获得大鼠骨髓来源单核细胞,接种在包被有大鼠玻连蛋白的培养皿中,加入内皮细胞生长培养基-2,于常规培养条件(37℃、5%CO2)下培养,第4天时,弃去未贴壁细胞,全量更换新鲜培养基,随机分为常规培养组和缺氧培养组,常规培养组于常规培养条件下继续培养至第7天;缺氧培养组分别于常规培养的第4、5、6天,于缺氧培养条件(1%O2+5%CO2+94%N2)下继续培养72、48、24 h;细胞表面标志物检测并Dil标记的乙酰化低密度脂蛋白和FITC标记的荆豆凝集素1共同染色方法鉴定BM-EPCs,分别对各组培养至第7天的细胞进行内皮分化功能检测(Matrigel As-say)和抗凋亡检测(Annexin V/PI)。结果随着缺氧处理时间延长,BM-EPCs早期凋亡率增加明显。与常规培养组(0.89±0.20)%相比,缺氧培养24 h组早期凋亡率(1.33±0.07)%改变不明显(P0.05),缺氧培养48 h组(3.25±0.12)%、72 h组(7.48±1.53)%,早期凋亡率明显增加(P0.05)。与常规培养组比较,缺氧培养24 h组的体外血管形成能力增强(P0.01),缺氧培养48、72 h组体外血管形成能力明显下降(P0.01)。结论缺氧预处理BM-EPCs24 h,BM-EPCs早期凋亡率增加不明显,细胞体外血管形成能力明显增强。
[Abstract]:Objective to study the effects of hypoxia on the biological function of bone marrow derived endothelial progenitor cells (BM-EPCs). Methods Mononuclear cells from rat bone marrow were obtained by density gradient centrifugation. The monocytes were inoculated in a culture dish coated with rat Glassin. The endothelial cell growth medium (-2) was added to the culture medium. The cells were cultured under conventional culture conditions (37 鈩，

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