大鼠颈上神经节细胞内局部钙信号的研究
发布时间：2018-08-19 20:19
【摘要】：Ca~(2+)是细胞内一种重要的信号分子,它在肌肉收缩、突触传递、激素分泌、基因转录、细胞的存活和死亡等众多重要的生理过程中发挥关键的作用。钙火花作为胞内钙释放的基本单元在心肌、平滑肌、骨骼肌等肌肉细胞上研究比较多,而在神经细胞上研究的比较少。颈上神经节是用来研究交感系统和突触传递的一个很好的标本,对于钙火花的研究还没有涉及过。 在本文的研究中,我们利用共聚焦显微镜在颈上交感神经节细胞上探测神经元钙火花的存在,并分析其特性,比较颈上神经节细胞与其它类型细胞钙火花的异同点。取出生3～7天的SD大鼠颈上神经节细胞做原代培养,实验前用Fluo-4-AM荧光探针事先装载培养了3～10天的细胞,在Zeiss 510倒置共聚焦显微镜下,用低浓度的caffeine作为胞内钙库的刺激物来诱发钙信号。我们发现,1.5mM的caffeine可以在细胞的胞体和神经突上诱发产生钙火花。对钙火花的参数分析发现,颈上神经节细胞的钙火花有它自身的特点:与经
[Abstract]:Ca2 is an important signal molecule in cells, which plays a key role in many important physiological processes, such as muscle contraction, synaptic transmission, hormone secretion, gene transcription, cell survival and cell death. As the basic unit of intracellular calcium release, calcium sparks are studied more in muscle cells, smooth muscle, skeletal muscle and other muscle cells, but less in nerve cells. The superior cervical ganglion is a good specimen for the study of sympathetic system and synaptic transmission, but has not been involved in the study of calcium sparks. In the present study, we use confocal microscope to detect the existence of calcium sparks in the superior cervical sympathetic ganglion cells, and analyze their characteristics, and compare the differences of calcium sparks between the superior cervical ganglion cells and other types of cells. The supracervical ganglion cells of SD rats were taken out for primary culture after 3 days old for 7 days. Before the experiment, the cells were cultured with Fluo-4-AM fluorescence probe for 10 days. The cells were cultured under Zeiss 510 inverted confocal microscope. Low concentration of caffeine was used as the stimulation of intracellular calcium reservoir to induce calcium signal. We found that 1.5 mm of caffeine could induce calcium sparks in the cell body and neuronal process. The analysis of the parameters of calcium sparks shows that the calcium sparks of the superior cervical ganglion cells have their own characteristics:
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33
本文编号：2192728
[Abstract]:Ca2 is an important signal molecule in cells, which plays a key role in many important physiological processes, such as muscle contraction, synaptic transmission, hormone secretion, gene transcription, cell survival and cell death. As the basic unit of intracellular calcium release, calcium sparks are studied more in muscle cells, smooth muscle, skeletal muscle and other muscle cells, but less in nerve cells. The superior cervical ganglion is a good specimen for the study of sympathetic system and synaptic transmission, but has not been involved in the study of calcium sparks. In the present study, we use confocal microscope to detect the existence of calcium sparks in the superior cervical sympathetic ganglion cells, and analyze their characteristics, and compare the differences of calcium sparks between the superior cervical ganglion cells and other types of cells. The supracervical ganglion cells of SD rats were taken out for primary culture after 3 days old for 7 days. Before the experiment, the cells were cultured with Fluo-4-AM fluorescence probe for 10 days. The cells were cultured under Zeiss 510 inverted confocal microscope. Low concentration of caffeine was used as the stimulation of intracellular calcium reservoir to induce calcium signal. We found that 1.5 mm of caffeine could induce calcium sparks in the cell body and neuronal process. The analysis of the parameters of calcium sparks shows that the calcium sparks of the superior cervical ganglion cells have their own characteristics:
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33
【引证文献】
相关博士学位论文 前1条
1 丁艳平;摘除颈上神经节对成年大鼠海马齿状回神经再生及学习记忆的影响[D];兰州大学;2008年
，本文编号：2192728
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