首次从我国蚊虫中分离到类似广平病毒

发布时间：2018-08-29 15:43

【摘要】：为掌握云南省西双版纳州勐海县蚊媒病毒分布状况,用诱蚊灯采集蚊虫标本,用细胞培养法分离病毒,对阳性分离物进行分子生物学鉴定。2012年7月,在勐海县打洛镇采集蚊虫32批共1 468只,其中三带喙库蚊(Culex tritaeniorhynchus)28批1 383只,致倦库蚊(Culex quinquefascitatus)2批66只,按蚊(Anopheles)2批19只。用金黄地鼠肾细胞(Golden hamster kidney cell,简称BHK-21)和白纹伊蚊细胞(Aedes albopictus cell,简称C6/36)进行病毒分离,结果有2批三带喙库蚊标本(BNDL1205和BNDL1227)能引起C6/36细胞产生聚合病变,而不能引起BHK-21细胞病变。用黄病毒属特异性引物对这2株阳性分离物进行RT-PCR,扩增得到800bp左右的条带并进行核苷酸序列测定。对核苷酸序列的系统进化分析表明,这2株病毒与分离自越南的广平病毒(Quang Binh virus,QBV)VN180株亲缘性最近,处在同一进化分支;与VN180株的核苷酸同源性分别为83.4%和82.9%,而与既往国内外分离的18株蚊虫黄病毒的核苷酸差异较大。结果表明BNDL1205和BNDL1227病毒为类似QBV,此为国内首次报道。
[Abstract]:In order to understand the distribution of mosquito-borne virus in Menghai County, Xishuangbanna Prefecture, Yunnan Province, mosquito samples were collected by mosquito trap lamp, virus was isolated by cell culture method, and the positive isolate was identified by molecular biology in July, 2012. A total of 1 468 mosquitoes were collected in 32 batches in Daluo Town, Menghai County, of which 1 383 were (Culex tritaeniorhynchus) 28 batches, 66 were Culex pipiens quinquefasciatus (Culex quinquefascitatus) 2 and 19 were Anopheles japonicus (Anopheles) 2 batches. The virus was isolated from golden hamster kidney cells (BHK-21) and Aedes albopictus cell (Aedes albopictus cell, (C6 / 36). The results showed that two batches of Culex tritaeniorhynchus (BNDL1205 and BNDL1227) could cause polymeric lesions of C6 / 36 cells, but not BHK-21 cells. The bands of 800bp were amplified by RT-PCR, and the nucleotide sequences were sequenced with the specific primers of yellow virus. Phylogenetic analysis of nucleotide sequences showed that the two viruses were closely related to the (Quang Binh virus,QBV) VN180 strain isolated from Vietnam and were in the same evolutionary branch. The nucleotide homology with VN180 strain was 83.4% and 82.9% respectively. The results showed that BNDL1205 and BNDL1227 viruses were similar to QBV, which was the first reported in China.
【作者单位】：云南省地方病防治所云南省病毒立克次体研究中心;西双版纳州疾病预防控制中心;中国疾病预防控制中心病毒病预防控制所;
【基金】：国家自然科学基金资助项目(No.30560142) 西双版纳州科技项目(201017)
【分类号】：R373.3

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