GM-CSF对诱导人血管内皮细胞血管形成的影响及VEGF的作用

发布时间：2018-09-04 11:28

【摘要】：前言动脉粥样硬化(atherosclerosis,AS)一直被认为是一种慢性进展的血管壁脂质沉积性疾病。近些年的研究逐渐阐明了炎症在AS病理过程中的重要作用,认为炎症可导致不稳定斑块形成,甚至斑块破裂,引发急性冠状动脉综合症(acute coronary syndrome,ACS)等临床严重并发症。而不稳定斑块的重要特征之一就是斑块内血管新生。血管新生是由既存的血管以出芽的方式形成新血管的过程,见于许多生理和病理情况,具有广泛的生物学和临床意义。血管新生的过程极其复杂,受到促进血管生成因子和抑制血管生成因子的共同调控。斑块内的新生血管可作为更多的血浆脂蛋白进入斑块的通道而促进斑块生长。更重要的是,通过新生血管,血液中大量炎细胞流入斑块内,产生多种炎性因子、细胞因子和生长因子,从而加重炎症反应。并且新生血管本身无平滑肌和周细胞支持,管壁薄弱,易于引起斑块不稳定、斑块内出血,甚至斑块破裂。目前,关于炎性因子粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)对血管新生的作用研究较少。因此,本实验在体外培养人脐带静脉内皮细胞(human umbilical vein endothelial cells,HUVECs),应用Matrigel建立稳定的血管形成的体外培养体系,来研究炎性因子GM-CSF对诱导人血管内皮细胞血管形成的影响及血管内皮生长因子(vascular endothelial growth factor,VEGF)的作用,进而探讨GM-CSF和VEGF与AS斑块稳定性及病变进展之间的关系。方法采用改进的Jaffe氏培养法进行HUVECs培养,应用Matrigel诱导内皮细胞形成管腔结构,从而建立稳定的血管形成的体外培养体系,然后施加实
[Abstract]:Preface atherosclerosis (atherosclerosis,AS) has been considered as a chronic progressive lipid deposition disease of vascular wall. In recent years, studies have gradually clarified the important role of inflammation in the pathological process of AS. It is believed that inflammation can lead to unstable plaque formation, even plaque rupture, acute coronary syndrome (acute coronary syndrome,ACS) and other serious clinical complications. One of the most important characteristics of unstable plaque is angiogenesis. Angiogenesis is the process of forming new blood vessels by budding of existing blood vessels, which can be seen in many physiological and pathological conditions and has extensive biological and clinical significance. The process of angiogenesis is very complicated and is regulated by both angiogenic factors and inhibiting angiogenic factors. The neovascularization in plaque can promote plaque growth as more plasma lipoproteins enter the plaque channel. More importantly, through neovascularization, a large number of inflammatory cells in the blood flow into the plaque, producing a variety of inflammatory factors, cytokines and growth factors, thereby exacerbating the inflammatory response. Moreover, the neovascularization itself has no smooth muscle and pericyte support, and the wall of the vessel is weak, which can easily lead to plaque instability, plaque hemorrhage and even plaque rupture. At present, there are few studies on the effect of inflammatory granulocyte-macrophage colony stimulating factor (granulocyte-macrophage colony stimulating factor,GM-CSF) on angiogenesis. Therefore, human umbilical vein endothelial cells (human umbilical vein endothelial cells,HUVECs) were cultured in vitro and Matrigel was used to establish a stable angiogenic culture system in vitro. To investigate the effect of inflammatory factor GM-CSF on the angiogenesis of human vascular endothelial cells and the effect of vascular endothelial growth factor (vascular endothelial growth factor,VEGF), and to explore the relationship between GM-CSF and VEGF and the stability of AS plaque and the progression of AS. Methods the improved Jaffe's culture method was used to culture HUVECs, and Matrigel was used to induce endothelial cells to form lumen structure, so as to establish a stable culture system of angiogenesis in vitro. And then apply the solid
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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