外周血单个核细胞在不同条件下HSP70形成的研究
发布时间：2018-09-05 09:18
【摘要】：目的:热休克蛋白(hot shock protein ,HSP)又称作分子伴侣,是所有细胞能在应激情况下所产生的一组蛋白质。根据分子量的大小分为 HSP20、HSP70、HSP90 和大分子 HSP 家族。HSP70 是 HSP 家族中最重要的一种。HSP70 被认为同时与新生的多肽链结合部分,HSP70 作为防止蛋白质的聚集及错误折叠的媒介。HSP70 与机体的生理作用密切相关,参与细胞内的许多功能,如蛋白质折叠、运转、成熟及降解。目前对 HSP 的研究成为热点,但 HSP 的确切作用还仍未了解,特别是介质对热休克蛋白形成的影响研究未见报道。本试验的目的是通过人外周血单个核细胞不同时间内在不同 pH 和不同介质中 HSP70 形成的研究,探讨影响热休克蛋白形成的因素。 方法:抽取无发烧、无炎症的健康志愿者外周血,应用密度梯度法分离单个核细胞,分别在不同 pH 的 AB 型血清和不同介质(1640 培养液、AB 型人血清、PBS 缓冲液)中 42℃水浴中热休克 0.5、1 小时,用免疫组化法测定热休克蛋白 70 的形成。 结果:外周血单个核细胞在不同pH的AB型血清中42℃热休克0.5、1 小时,热休克蛋白 70 的表达与未作热休克处理的空白比较有明显差异,P0.01。外周血单个核细胞在 AB 血清中(pH7.35)42℃热休克0.5、1 小时热休克蛋白 70 的形成有明显差异,P0.01。热休克 0.5小时的表达明显高于 1 小时;酸性(pH6.95)AB 血清中 42℃热休克 0.5、1 小时热休克蛋白 70 的形成无明显差异,P0.05;碱性(pH7.65)AB血清中 42℃热休克 0.5、1 小时热休克蛋白 70 的形成无明显差异,P 0.05。 外周血单个核细胞在不同介质中 42℃热休克 0.5、1 小时,热休克蛋白 70 的表达与空白比较有显著性差异,P0.01。在磷酸盐缓冲液中42℃热休克0.5、1小时,热休克蛋白70的形成有明显差异,P0.01,0.5 小时的表达高于 1 小时;1640 培养液中 42℃热休克 0.5、1 小时,热休克蛋白 70 的表达差异不明显,P0.05。 结论:(1)外周血单个核细胞热休克蛋白 70 形成的量与应激源数量密切相关,多种应激源刺激热休克蛋白质 70 形成的量比单一应激源刺激形成的快。(2)碱性条件下外周血单个核细胞也可产生热休克蛋白。(3)外周血单个核细胞热休克蛋白的形成与细胞所处的介质密切相关。
[Abstract]:Objective: heat shock protein (hot shock protein), also known as molecular chaperone, is a group of proteins produced by all cells under stress. HSP20,HSP70,HSP90 and macromolecule HSP family. HSP70 is the most important type of HSP family. HSP70 is considered to be the medium to prevent protein aggregation and misfolding. Closely related to the physiological function of the body, Participate in many cellular functions, such as protein folding, translocation, maturation, and degradation. At present, the research on HSP has become a hot topic, but the exact role of HSP has not been understood, especially the effect of medium on the formation of heat shock proteins has not been reported. The purpose of this study was to investigate the factors affecting the formation of heat shock proteins (HSPs) in human peripheral blood mononuclear cells (PBMCs) by studying the formation of HSP70 in different pH and medium at different time. Methods: mononuclear cells were isolated from the peripheral blood of healthy volunteers without fever and inflammation by density gradient method. The formation of heat shock protein 70 (HSP70) was determined by immunohistochemical method in AB type serum of different pH and in different medium (1640 medium) in water bath at 42 鈩，
本文编号：2223848
[Abstract]:Objective: heat shock protein (hot shock protein), also known as molecular chaperone, is a group of proteins produced by all cells under stress. HSP20,HSP70,HSP90 and macromolecule HSP family. HSP70 is the most important type of HSP family. HSP70 is considered to be the medium to prevent protein aggregation and misfolding. Closely related to the physiological function of the body, Participate in many cellular functions, such as protein folding, translocation, maturation, and degradation. At present, the research on HSP has become a hot topic, but the exact role of HSP has not been understood, especially the effect of medium on the formation of heat shock proteins has not been reported. The purpose of this study was to investigate the factors affecting the formation of heat shock proteins (HSPs) in human peripheral blood mononuclear cells (PBMCs) by studying the formation of HSP70 in different pH and medium at different time. Methods: mononuclear cells were isolated from the peripheral blood of healthy volunteers without fever and inflammation by density gradient method. The formation of heat shock protein 70 (HSP70) was determined by immunohistochemical method in AB type serum of different pH and in different medium (1640 medium) in water bath at 42 鈩，
本文编号：2223848
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