Toll受体mRNA在应用抗生素大鼠体内的表达及有关因素相关分析的研究
发布时间：2018-09-05 13:29
【摘要】： 目的:抗生素所致腹泻是抗生素应用过程中发生的一种副作用。有实验证明抗生素应用可以引起肠道菌群结构紊乱,细菌易位现象发生及免疫功能受到抑制。本实验采用盐酸林可霉素制造动物腹泻模型,采用大肠杆菌、活菌制剂干预,其目的是①采用细菌培养、鉴定、G染色及厌氧菌代谢产物的气相色谱分析等方法动态观察抗生素致腹泻大鼠3-14天的肠道菌群结构及远隔器官的细菌易位现象。从微生态角度出发解释细菌易位现象的发生机制。②利用反转录聚合酶链反应(RT-PCR)技术测定抗生素致腹泻大鼠肠粘膜组织、肠系膜淋巴结(MLN)、肝脏、脾脏中Toll-like recepters (TLRs)mRNA的表达。说明抗生素应用对模式识别受体机制(pattern recognition receptors mechanism,PRRs机制)的影响,分析抗生素应用,细菌易位及免疫功能的关系。探讨模式识别受体机制在机体防御机制:天然免疫和获得性免疫中的重要作用。③采用活菌制剂乳酸杆菌灌胃的方法对比观察腹泻后菌群结构、细菌易位及免疫功能的变化,以了解活菌制剂的干预效应。④采用条件致病菌埃希氏致病性大肠杆菌灌胃的方法对比观察细菌易位、肠道免疫功能的变化,以了解抗生素应用造成的二重感染对细菌易位和免疫功能的影响。 方法:采用健康SD大鼠60只,雌雄各半,体重220-240
[Abstract]:Objective: antibiotic-induced diarrhea is a side effect of antibiotic application. Some experiments have proved that the use of antibiotics can cause intestinal microflora structure disorder, bacterial translocation phenomenon and the inhibition of immune function. In this experiment, lincomycin hydrochloride was used to make animal diarrhea model, Escherichia coli and live bacteria preparation were used to intervene. The purpose of this experiment was to use bacteria culture. The intestinal flora structure and bacterial translocation in distant organs of antibiotic induced diarrhea rats were dynamically observed by means of G staining and gas chromatographic analysis of anaerobes metabolites. The mechanism of bacterial translocation was explained from the microecological point of view. The expression of Toll-like recepters (TLRs) mRNA in intestinal mucosa, mesenteric lymph node, liver and spleen of rats with antibiotic induced diarrhea was determined by reverse transcriptase polymerase chain reaction (RT-PCR). The effect of antibiotic application on the (pattern recognition receptors mechanism,PRRs mechanism of pattern recognition receptor was explained, and the relationship among antibiotic application, bacterial translocation and immune function was analyzed. To explore the important role of pattern recognition receptor mechanism in the defense of organism: .3 the changes of bacterial community structure, bacterial translocation and immune function after diarrhea were observed by the method of intragastric perfusion of Lactobacillus, a living bacterium preparation, in innate and acquired immunity. In order to understand the intervention effect of living bacteria preparation, the method of oral administration of Escherichia coli was used to observe the changes of bacterial translocation and intestinal immune function. To understand the effect of double infection caused by antibiotic application on bacterial translocation and immune function. Methods: 60 healthy SD rats, half male and female, weight 220-240 were used.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392
本文编号：2224413
[Abstract]:Objective: antibiotic-induced diarrhea is a side effect of antibiotic application. Some experiments have proved that the use of antibiotics can cause intestinal microflora structure disorder, bacterial translocation phenomenon and the inhibition of immune function. In this experiment, lincomycin hydrochloride was used to make animal diarrhea model, Escherichia coli and live bacteria preparation were used to intervene. The purpose of this experiment was to use bacteria culture. The intestinal flora structure and bacterial translocation in distant organs of antibiotic induced diarrhea rats were dynamically observed by means of G staining and gas chromatographic analysis of anaerobes metabolites. The mechanism of bacterial translocation was explained from the microecological point of view. The expression of Toll-like recepters (TLRs) mRNA in intestinal mucosa, mesenteric lymph node, liver and spleen of rats with antibiotic induced diarrhea was determined by reverse transcriptase polymerase chain reaction (RT-PCR). The effect of antibiotic application on the (pattern recognition receptors mechanism,PRRs mechanism of pattern recognition receptor was explained, and the relationship among antibiotic application, bacterial translocation and immune function was analyzed. To explore the important role of pattern recognition receptor mechanism in the defense of organism: .3 the changes of bacterial community structure, bacterial translocation and immune function after diarrhea were observed by the method of intragastric perfusion of Lactobacillus, a living bacterium preparation, in innate and acquired immunity. In order to understand the intervention effect of living bacteria preparation, the method of oral administration of Escherichia coli was used to observe the changes of bacterial translocation and intestinal immune function. To understand the effect of double infection caused by antibiotic application on bacterial translocation and immune function. Methods: 60 healthy SD rats, half male and female, weight 220-240 were used.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392
【参考文献】
相关期刊论文 前1条
1 李红云,姚咏明,盛志勇;Toll样受体与脓毒症的研究进展[J];中华烧伤杂志;2002年05期
，本文编号：2224413
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