婴幼儿致病性大肠埃希菌的毒力基因检测研究

发布时间：2018-09-08 13:06

【摘要】：目的通过对婴幼儿腹泻病例粪便标本进行致病性大肠埃希菌的分离鉴定及毒力基因检测,从分子生物学方面研究致病性大肠埃希菌引起婴幼儿腹泻的分布,以降低婴幼儿致病性大肠埃希菌的感染率。方法收集江西省儿童医院2011-2012年婴幼儿腹泻病例357份粪便标本,按照致病性大肠埃希菌分离鉴定程序用EC肉汤增菌过夜,增菌后用麦康凯琼脂培养基分离培养,可疑菌用API 20E生化鉴定出大肠埃希菌,然后用聚合酶链反应(PCR)检测致病性大肠埃希菌相应的毒力基因。结果 357份腹泻患者粪便标本中,检出37份携带毒力基因的致病性大肠埃希菌,检出率为10.36%,携带毒力基因的肠聚集性大肠埃希菌(EAggEC)、肠道致病性大肠埃希菌(EPEC)、肠道侵入性大肠埃希菌(EIEC)、肠道出血性大肠埃希菌(EHEC)检出率分别为8.4%、0.56%、1.12%、0.28%,产肠毒素大肠埃希菌(ETEC)未检出。结论婴幼儿腹泻病例中致病性大肠埃希菌主要由EaggEC引起,其次为EIEC,进一步证实对致泻大肠埃希菌进行PCR毒力基因检测具有重要临床意义。
[Abstract]:Objective To study the distribution of pathogenic Escherichia coli causing infantile diarrhea from molecular biology through isolation and identification of pathogenic Escherichia coli from feces of infantile diarrhea cases and detection of virulent genes in order to reduce the infection rate of pathogenic Escherichia coli in infants and young children. 357 stool specimens of infantile diarrhea cases were cultured in McConkey agar medium after the addition of pathogenic Escherichia coli by EC broth. The suspicious bacteria were identified by API 20E biochemistry. The virulence genes of pathogenic Escherichia coli were detected by polymerase chain reaction (PCR). Results Among the 357 stool specimens of diarrhea patients, 37 pathogenic Escherichia coli carrying virulent genes were detected, the detection rate was 10.36%. The detection rates of enteric aggregated Escherichia coli (EAggEC), enteropathogenic Escherichia coli (EPEC), enteric invasive Escherichia coli (EIEC) and enterohemorrhagic Escherichia coli (EHEC) carrying virulent genes were respectively. Enterotoxigenic Escherichia coli (ETEC) was not detected in 8.4%, 0.56%, 1.12%, 0.28%. Conclusion The pathogenic Escherichia coli in infantile diarrhea cases was mainly caused by EaggEC, followed by EIEC. It was further confirmed that PCR virulence gene detection of diarrhogenic Escherichia coli had important clinical significance.
【作者单位】：江西省疾病预防控制中心传染病预防控制所;
【基金】：国家科技重大专项基金项目(2013zx10004-203-002)
【分类号】：R378.21

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